Lab Anim Res.  2015 Sep;31(3):101-110. 10.5625/lar.2015.31.3.101.

Hepatotoxicity and nephrotoxicity of gallotannin-enriched extract isolated from Galla Rhois in ICR mice

Affiliations
  • 1Department of Biomaterials Science, College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, Korea. dyhwang@pusan.ac.kr
  • 2Department of Clinical Laboratory Science, College of Nursing and Healthcare Science, Dong-Eui University, Busan, Korea.

Abstract

To evaluate the hepatotoxicity and nephrotoxicity of Galla Rhois (GR) toward the liver and kidney of ICR mice, alterations in related markers including body weight, organ weight, urine composition, liver pathology and kidney pathology were analyzed after oral administration of 250, 500 and 1,000 mg/kg body weight/day of gallotannin-enriched extract of GR (GEGR) for 14 days. GEGR contained 68.7+/-2.5% of gallotannin, 25.3+/-0.9% of gallic acid and 4.4+/-0.1% of methyl gallate. Also, the level of malondialdehyde (MDA), a marker of lipid peroxidation, was decreased with 19% in the serum of high dose GEGR (HGEGR)-treated mice. The body and organ weight, clinical phenotypes, urine parameters and mice mortality did not differ among GEGR-treated groups and the vehicle-treated group. Furthermore, no significant increase was observed in alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), blood urea nitrogen (BUN) and the serum creatinine (Cr) in the GEGR-treated group relative to the vehicle-treated group. Moreover, the specific pathological features induced by most toxic compounds such as CCl4 were not observed upon liver and kidney histological analysis. Overall, the results of the present study suggest that GEGR does not induce any specific toxicity in liver and kidney organs of ICR at doses of 1,000 mg/kg body weight/day, indicating that this is no observed adverse effect level (NOAEL).

Keyword

Galla Rhois; hepatotoxicity; nephrotoxicity; gallotannin; histopathology

MeSH Terms

Administration, Oral
Alanine Transaminase
Alkaline Phosphatase
Animals
Aspartate Aminotransferases
Blood Urea Nitrogen
Body Weight
Creatinine
Gallic Acid
Kidney
L-Lactate Dehydrogenase
Lipid Peroxidation
Liver
Malondialdehyde
Mice
Mice, Inbred ICR*
Mortality
No-Observed-Adverse-Effect Level
Organ Size
Pathology
Phenotype
Alanine Transaminase
Alkaline Phosphatase
Aspartate Aminotransferases
Creatinine
Gallic Acid
L-Lactate Dehydrogenase
Malondialdehyde
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