Korean J Physiol Pharmacol.  2014 Aug;18(4):321-326. 10.4196/kjpp.2014.18.4.321.

Protective Effects of the Nuclear Factor Kappa B Inhibitor Pyrrolidine Dithiocarbamate on Experimental Testicular Torsion and Detorsion Injury

• Kabay S
• Ozden H
• Guven G
• Burukoglu
• Ustuner MC
• Topal F
• Gunes HV
• Ustuner D
• Ozbayer C

• KMID: 2285526
• DOI: http://doi.org/10.4196/kjpp.2014.18.4.321

Abstract

Testicular torsion results with the damage of the testis and it is a surgical emergency. Pyrrolidine dithiocarbamate (PDTC) is a low-molecular-weight antioxidant and potent inhibitor of nuclear factor kappa B (NF-kappaB) activation. In this study, we aimed to investigate the effects of PDTC to testicular torsion-detorsion (T/D) injury. Forty adult male Sprague-Dawley rats were separated into four groups. A sham operation was performed in group I. In group II, torsion is performed 2 hours by 720 degree extravaginally testis. In group III, 4 h reperfusion of the testis was performed after 2 h of testicular torsion. In group IV, after performing the same surgical procedures as in group III, PDTC (100 mg/kg, intravenous's) was administered before 30 min of detorsion. The testes tissue malondialdehyde (MDA), superoxide dismutase (SOD) catalase (CAT) level was evaluated. Histological evaluations were performed after hematoxylin and eosin staining. Testicular tissue MDA levels were the highest in the T/D groups compared with treatment group. Administration of PDTC prevented a further increase in MDA levels. Significant decrease occurred in CAT and SOD levels in treatment group compared with the control group. The rats in the treatment group had normal testicular architecture. The results suggest that PDTC can be a potential protective agent for preventing the biochemical and histological changes related to oxidative stress in testicular injury caused by testis torsion.

Keyword

Antioxidant enzyme; Ischemia-reperfusion; Pyrrolidine dithiocarbamate; Testicular torsion

MeSH Terms

Adult
Animals
Catalase
Cats
Emergencies
Eosine Yellowish-(YS)
Hematoxylin
Humans
Male
Malondialdehyde
NF-kappa B*
Oxidative Stress
Rats
Rats, Sprague-Dawley
Reperfusion
Spermatic Cord Torsion*
Superoxide Dismutase
Testis
Catalase
Eosine Yellowish-(YS)
Hematoxylin
Malondialdehyde
NF-kappa B
Superoxide Dismutase

Figure

• 

  Fig. 1 Mean MDA, SOD, CAT activities of all groups.

• 

  Fig. 2 (A) Sham group shows normal testicular architecture and regular seminiferous tubular morphology with normal spermatogenesis (star) (H&E, 50×). (B) Torsion group shows severe tubular degeneration, many of the tubules of spermatogenic cell lines completely disappeared (black arrows), thickening of the basal laminae of tubules (yellow arrows), Interstitial edema (stars) and vascular congestions, hemorrhage (black arrowhead) in the area, some of the tubules has necrotic (pyknotic nuclei and eosinophilic cytoplasm) cells spermatocytes (white arrows) (H&E, 20×, 50×). (C) Torsion/Detorsion group shows tubule lumen be filled with many cellular desquamation, degeneration of germ cells (stars), severe tubular degeneration (black arrowhead), necrotic cells (white arrowhead) and eosinophilic accumulation of fluid in the interstitial space and vascular congestions (black arrows) (H&E, 20×, 100×). (D) The PDTC-treated group shows maturation up to the level of spermatozoa, with preservation of tubular morphology (H&E, 20×).

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