Korean J Nephrol.  2008 May;27(3):341-347.

The Association of KLOTHO Gene Polymorphism with Serum Osteoprotegerin Levels in Maintenance Hemodialysis Patients

• Lee YS
• Kim H
• Kim HS
• Lee KB

Abstract

PURPOSE
Klotho mutant mice showed abnormal calcium and vitamin D metabolism, hyperphosphatemia and vascular calcification. We observed the frequencies of klotho gene polymorphism and investigated their relation with some clinical parameters including serum osteoprotegerin (OPG) levels in maintenance hemodialysis (HD) patients.
METHODS
Total 88 patients (mean age 58+/-13 years, male:female=47:41) on maintenance HD were enrolled. The genotypings for G-395A in promoter and C1818T in exon 4 of klotho gene were performed with real-time polymerase chain reaction. We measured blood pressure, body mass index (BMI), and serum calcium, phosphorus, parathyroid hormone (PTH), alkaline phosphatase, hs-CRP, lipid profiles and OPG.
RESULTS
In G-395A in promoter, the distribution of genotypes was GG 66% (n=58), GA 23% (n=20) and AA 11% (n=10), respectively and the allele frequencies were 0.773 for G allele and 0.227 for A allele. In C1818T in exon 4, the distribution of genotypes was CC 63% (n=55), CT 30% (n=26), and TT 7% (n=7), and the frequencies were 0.773 for C allele and 0.227 for T allele. G-395A shows correlations with BMI and HDL-cholesterol (p<0.005). G-395A and C1818T in klotho gene show no statistical correlation with other clinical parameters of vascular calcification including OPG.
CONCLUSION
Klotho G-395A and C1818T polymorphisms are not correlated with OPG in maintenance HD patients. Further research needs for the other klotho polymorphisms on chronic kidney disease and end-stage renal disease.

Keyword

Klotho protein; Osteoprotegerin; Hemodialysis

MeSH Terms

Alkaline Phosphatase
Alleles
Animals
Blood Pressure
Body Mass Index
Calcium
Exons
Gene Frequency
Genotype
Glucuronidase
Humans
Hyperphosphatemia
Mice
Osteoprotegerin
Parathyroid Hormone
Phosphorus
Real-Time Polymerase Chain Reaction
Renal Dialysis
Renal Insufficiency, Chronic
Vascular Calcification
Vitamin D
Alkaline Phosphatase
Calcium
Glucuronidase
Osteoprotegerin
Parathyroid Hormone
Phosphorus
Vitamin D