- Real-Time Polymerase Chain Reaction Assay for the Diagnosis of Pulmonary Tuberculosis
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Wiwanitkit V
- KMID: 2320731
- Tuberc Respir Dis.
- 2015 Oct;78(4):473-473.
- doi: 10.4046/trd.2015.78.4.473
No abstract available. -
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- Real-Time Polymerase Chain Reaction Assay: A Response to Recent Letter to the Editor
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Kim SW, Chang JH
- KMID: 2320756
- Tuberc Respir Dis.
- 2016 Jan;79(1):46-46.
- doi: 10.4046/trd.2016.79.1.46
No abstract available. -
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- Real-time Polymerase Chain Reaction (PCR) in Microbiology
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Lee MK
- KMID: 2170099
- Infect Chemother.
- 2004 Apr;36(2):105-113.
Real-time polymerase chain reaction (PCR) is based on the detection and quantification of a fluorescent reporter. The signal increases in direct proportion to the amount of PCR product formed during... -
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- Comparison between Real-Time PCR and Agarose Gel Electrophoresis for DNA Quantification
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Lee MK, Kim HR
- KMID: 2238890
- Korean J Lab Med.
- 2006 Jun;26(3):217-222.
- doi: 10.3343/kjlm.2006.26.3.217
BACKGROUND: Real-time polymerase chain reaction (PCR) is generally regarded as a very accurate and time-saving method, but it is expensive to run. We evaluated the reliability of an inexpensive and... -
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- Detection of Enterovirus in Cerebrospinal Fluid by Real-Time Nested Reverse Transcription Polymerase Chain Reaction
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Heo SR, Jin SK, Chang HE, Park KU, Song J, Kim EC
- KMID: 2143190
- Korean J Lab Med.
- 2006 Feb;26(1):9-13.
- doi: 10.3343/kjlm.2006.26.1.9
BACKGROUND: Enterovirus is a common cause of aseptic meningitis, respiratory disease and nonspecific febrile illness. The conventional methods for laboratory diagnosis of enterovirus infections have been virus culture and serotyping... -
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- Real-time Reverse Transcription Polymerase Chain Reaction Using Total RNA Extracted from Nasopharyngeal Aspirates for Detection of Pneumococcal Carriage in Children
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Kim YK, Lee KH, Yun KW, Lee MK, Lim IS
- KMID: 2362478
- Pediatr Infect Vaccine.
- 2016 Dec;23(3):194-201.
- doi: 10.14776/piv.2016.23.3.194
PURPOSE: Monitoring pneumococcal carriage rates is important. We developed and evaluated the accuracy of a real-time reverse transcription polymerase chain reaction (RT-PCR) protocol for the detection of Streptococcus pneumoniae. METHODS: In... -
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- Diagnosis of Viral Infection Using Real-time Polymerase Chain Reaction
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Hwang KA, Ahn JH, Nam JH
- KMID: 2409049
- J Bacteriol Virol.
- 2018 Mar;48(1):1-13.
- doi: 10.4167/jbv.2018.48.1.1
The laboratory-based diagnosis of viral infection has been evolving over the years, to increase objectivity, accuracy, and sensitivity via the continuous development of various technologies. Cell culture, which is one... -
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- Peptoniphilus mikwangii-specific quantitative real-time polymerase chain reaction primers
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Park SN, Kook JK
- KMID: 2460805
- Int J Oral Biol.
- 2019 Sep;44(3):96-100.
- doi: 10.11620/IJOB.2019.44.3.96
The purpose of this study was to develop Peptoniphilus mikwangii -specific quantitative real-time polymerase chain reaction (qPCR) primers based on the 16S ribosomal RNA (16S rDNA) gene. The specificity of... -
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- Comparison of Multiplex Real-Time Polymerase Chain Reaction Assays for Detection of Respiratory Viruses in Nasopharyngeal Specimens
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Uwizeyimana JD, Kim MK, Kim D, Byun JH, Yong D
- KMID: 2450001
- Ann Clin Microbiol.
- 2019 Jun;22(2):35-41.
- doi: 10.5145/ACM.2019.22.2.35
BACKGROUND: Respiratory tract infections are major public health threats, and the identification of their causative microbes helps clinicians to initiate timely and appropriate antimicrobial therapy and prevent the secondary spread... -
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- Comparison Cytomegalovirus Qualitative Assay Using Real-Time PCR and Conventional PCR
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Jeong S, Kim YJ, Bae IK, Kim MJ, Jeong SH
- KMID: 2169598
- Ann Clin Microbiol.
- 2013 Mar;16(1):19-24.
- doi: 10.5145/ACM.2013.16.1.19
BACKGROUND: Cytomegalovirus (CMV) infection is a major cause of morbidity and mortality in immunocompromised patients. We compared the abilities of the recently developed Real-Q Cytomegalovirus Kit (Biosewoom Inc., Korea) and... -
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- Selective detection of viable Enterococcus faecalis using propidium monoazide in combination with real-time PCR
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Kim S, Lee S, Kim E, Seo D, Song Y, Jung I
- KMID: 1428464
- J Korean Acad Conserv Dent.
- 2008 Nov;33(6):537-544.
- doi: 10.5395/JKACD.2008.33.6.537
Polymerase chain reaction (PCR) can detect bacteria more rapidly than conventional plate counting. However DNA-based assays cannot distinguish between viable and dead cells due to persistence of DNA after cells... -
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- Cyclooxgenase-2 Expression in Chronic Inflammatory Bowel Disease:Comparing of Immunohistochemical Stain to Real-time PCR
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Park YS, Kim NI, Lee JS, Choi JH, Im CY, Jun DW, Jo YJ, Kim SH, Son BK, Lee WM, Kim EK
- KMID: 2174094
- Intest Res.
- 2008 Dec;6(2):128-134.
BACKGROUND/AIMS: This study compared the results of cyclooxygenase-2 (COX-2) expression in inflammatory bowel disease and tuberculous colitis as evident by immunochemical staining and real time polymerase chain reaction (PCR). METHODS:... -
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- Evaluation of Clinical Sample for Accupower UU Real-Time PCR Kit
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Min SK, Kim SK, Kim YS, Cho IC, Lee GI
- KMID: 1732622
- Korean J Urogenit Tract Infect Inflamm.
- 2014 Oct;9(2):99-103.
- doi: 10.14777/kjutii.2014.9.2.99
PURPOSE: In recent years, various diagnostic methods, including culture, immunological detection, conventional polymerase chain reaction (PCR) based methods, and microarray experiment have been applied for detection of Mycoplasma genitalium and... -
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- Supraclavicular BCG Lymphadenitis Noted at 21 Months after BCG Vaccination Confirmed by a Molecular Method
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Lee MH, Chae MH, Park KU, Cho HK
- KMID: 1720817
- Korean J Pediatr Infect Dis.
- 2014 Aug;21(2):139-143.
Bacille Calmette-Guerin (BCG) lymphadenitis is the most common complication of BCG vaccination. It commonly occurs in infants aged -
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- Validity of the New Caries Activity Test using Real-Time Polymerase Chain Reaction
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Kwon D, Kim H, Nam O, Kim M, Choi S, Kim K, Lee H
- KMID: 2438322
- J Korean Acad Pediatr Dent.
- 2018 Aug;45(3):354-362.
- doi: 10.5933/JKAPD.2018.45.3.354
Periogen is a new caries activity test using real-time polymerase chain reaction. The aim of this study was to assess the validity of Periogen by evaluating the correlation with dmft,... -
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- Performance Evaluation of Anyplex plus MTB/NTM and AdvanSure TB/NTM for the Detection of Mycobacterium tuberculosis and Nontuberculous Mycobacteria
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Choe W, Kim E, Park SY, Chae JD
- KMID: 1971111
- Ann Clin Microbiol.
- 2015 Jun;18(2):44-51.
- doi: 10.5145/ACM.2015.18.2.44
BACKGROUND: Polymerase chain reaction (PCR) methods from direct specimen are widely used for the rapid and accurate detection of mycobacteria infection. In this study, we evaluated two domestically developed detection... -
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- Rapid Identification of Rickettsiae using the Real-Time PCR
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Park HS, Lee JH, Jin KH, Jang WJ, Park KH, Kook YH, Lee SH
- KMID: 1483962
- J Bacteriol Virol.
- 2008 Dec;38(4):221-226.
- doi: 10.4167/jbv.2008.38.4.221
In this study, new real-time PCR method based on the groEL gene was developed and investigated. Four spotted fever group (SFG) strains, four typhus group (TG) strains, and four scrub... -
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- Evaluation of two Real-Time PCR Assays for the Detection of Chlamydia trachomatis
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Lee EY, Kim S, Kim HS
- KMID: 1848732
- J Lab Med Qual Assur.
- 2011 Jun;33(1):43-47.
BACKGROUND: Chlamydia trachomatis is common etiological agent of sexual transmitted disease. We evaluated two real-time PCR assays for the detection of C. trachomatis; Cobas TaqMan CT test v2.0 (Roche Diagnostics,... -
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- Comparative Evaluation of Multiplex Real-Time PCR Assays for Six Pathogens of Sexually Transmitted Infections
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Chung HS, Lee M
- KMID: 2372998
- Ann Clin Microbiol.
- 2017 Mar;20(1):1-6.
- doi: 10.5145/ACM.2017.20.1.1
BACKGROUND: The multiplex real-time PCR assay is a sensitive test for simultaneous detection of various pathogens of sexually transmitted infections (STIs). We evaluated the performance of two multiplex real-time PCR... -
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- Rapid and accurate diagnosis of Clostridium difficile infection by real-time polymerase chain reaction
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Song PH, Min JH, Kim YS, Jo SY, Kim EJ, Lee KJ, Lee J, Sung H, Moon JS, Whang DH
- KMID: 2402654
- Intest Res.
- 2018 Jan;16(1):109-115.
- doi: 10.5217/ir.2018.16.1.109
BACKGROUND/AIMS: The incidence and severity of Clostridium difficile infection (CDI) have increased worldwide, resulting in a need for rapid and accurate diagnostic methods. METHODS: A retrospective study was conducted to compare... -
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