Korean J Dermatol.  2014 Jan;52(1):1-6.

Effect of Horse Oil on Anti-bacterial, Inflammatory Cytokines, and Type I Collagen Expressions in Human HaCaT Keratinocytes and Fibroblasts

• Choi KH
• Lee YS
• Yoon JH
• Yoo WK
• Kim MR
• Lee KS
• Cho JW

Abstract

BACKGROUND
Anti-inflammation, anti-bactericidal, and collagen synthesis are important for health skin conditions. However, the effect of horse oil on anti-inflammation, anti-bactericidal, and collagen synthesis is largely unknown.
OBJECTIVE
The aim of this study was to evaluate the anti-bactericidal, anti-inflammatory, and synthesis of type I collagen of horse oil.
METHODS
Anti-bacterial effect was evaluated by disc diffusion test. Expressions of inflammatory cytokines were studied by RT-PCR analysis, real time PCR. Type I collagen expression was evaluated by Western blot in human HaCaT kertinocytes and fibroblasts.
RESULTS
Our data showed that horse oil exerted anti-bacterial effect on P.acnes and S.aureus. Expression of IL-10 was increased by horse oil-treated HaCaT cells. In addition, increased expression of type I collagen was observed in horse oil-treated human skin fibroblasts.
CONCLUSION
Horse oil exerts an anti-bactericidal effect against P.acnes and S.aureus. In addition, anti-inflammatory and anti-aging effects of horse oil will be mediated by up-regulation of IL-10 and type I collagen, respectively.

Keyword

Anti-bactericidal effect; Horse oil; IL-10; Type I collagen

MeSH Terms

Blotting, Western
Collagen
Collagen Type I*
Cytokines*
Diffusion
Fibroblasts*
Horses*
Humans*
Interleukin-10
Keratinocytes*
Real-Time Polymerase Chain Reaction
Skin
Up-Regulation
Collagen
Collagen Type I
Cytokines
Interleukin-10