J Korean Rheum Assoc.  1999 Jun;6(2):110-116.

Inhibition of Fas-mediated Apoptosis of Rheumatoid Synovial Cells by TGF-beta and IL-1 beta

Affiliations
  • 1Department of Medicine, University of Ulsan, Asan Medical Center, Seoul, Korea.
  • 2Asan Institute for Life Sciences, Seoul, Korea.

Abstract


OBJECTIVE
To investigate the proliferative and anti-apoptotic effects of TGF-beta and IL-1beta on rheumatoid synovial cells.
METHODS
Synovial cells obtained from surgical procedure of the rheumatoid joint were cultured with TFG-beta and IL-1beta The proliferative response of synovial cells was examined by non-radioactive cell proliferation assay. Fas-demiated apoptosis of synovial cells was measured by flow cytometry after addition of anti-Fas antibody.
RESULTS
TGF-beta and IL-1betaproliferated synovial cells in a dose-dependent manner. They also made synovial cells resistant to anti-Fas antibody induced apoptosis.
CONCLUSION
TGF-beta and IL-1betapromotes synovial cell proliferation possibly through interference with Fas-mediated apoptosis, suggesting their role in synovial hyperplasis in rheumatoid arthritis.

Keyword

Rheumatoid synovium; Apoptosis; TFG-beta; IL-1beta
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