Int J Oral Biol.
2011 Jun;36(2):79-82.
Strain-specific PCR Primers for the Detection of Prevotella intermedia ATCC 49046
- Affiliations
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- 1Department of Oral Biochemistry, School of Dentistry, Chosun University, 375 Seosuk-Dong, Dong-Ku, Gwangju 501-759, Korea. jkkook@chosun.ac.kr
- 2Department of Conservative Dentistry, School of Dentistry, Chosun University, 375 Seosuk-Dong, Dong-Ku, Gwangju 501-759, Korea.
- 3Department of Dental Hygiene, Songwon University College, 365 Songha-dong, Nam-gu, Gwangju, Korea.
Abstract
- The aim of this study was to develop Prevotella intermedia ATCC 49046-specific PCR primers designed based on the nucleotide sequence of a DNA probe Pig28. The strain-specificity of the PCR primers, Pig28-F1/Pig28-R1, was confirmed with 9 strains of P. intermedia and 25 strains (15 species) of Prevotella species. The detection limit of the PCR primers was 2 pg of the purified genomic DNA of P. intermedia ATCC 49046. These PCR primers were found to be useful for identifying P. intermedia ATCC 49046, particularly for determining the authenticity of the strain.
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