J Korean Acad Periodontol.
2002 Jun;32(2):281-290. 10.5051/jkape.2002.32.2.281.
Study on development of DNA probe for identification of Prevotella intermedia G8-9K-3
- Affiliations
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- 1Department of Periodontology, College of Dentistry, Chosun University, Korea.
- 2Department of preventive Dentistry, College of Dentistry, Chosun University, Korea.
- 3Department of Oral Biochemistry, College of Dentistry, Chosun University, Korea.
- KMID: 2057040
- DOI: http://doi.org/10.5051/jkape.2002.32.2.281
Abstract
- The purpose of this study is to develop species-specific DNA probe for detection and identification of Prevotella intermedia (P. intermedia) G8-9K-3. This study procedure includes (1) whole-genomic DNA extraction of P. intermedia G8-9K-3 (2) construction of the genomic DNA library, (3) screening of strain-specific DNA probe by reverse dot hybridization, (4) confirmation of strain-specific DNA probe by Southern blot hybridization, (5) determination of nucleotide sequences of strain-specific DNA probe. Twenty-eight recombinant plasmids containing Hind III-digested DNA fragments of P. intermedia G8-9K-3 were obtained. Reverse dot Hybridization and Southern blot analysis data showed that one of them, Pig3, could be P. intermedia G8-9K-3-specific DNA probe. This datum indicates that this Pig3 DNA probe could be useful in detection and identification of the P. intermedia G8-9K-3 strain.
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