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Application of 16S rRNA Gene-Targeted Next-Generation Sequencing for Bacterial Pathogen Detection in Continuous Ambulatory Peritoneal Dialysis Peritonitis

Kim YA, Kang EW, Moon HS, Kim D, Yong D

BACKGROUND: 16S rRNA gene-targeted next-generation sequencing (NGS) can detect microorganisms in a comprehensive reference database. To date, NGS has been successfully applied to samples such as urine, blood, and synovial...
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Minocycline and Its Impact on Microbial Dysbiosis in the Skin and Gastrointestinal Tract of Acne Patients

Thompson K, Rainer BM, Antonescu , Florea L, Mongodin E, Kang S, Chien AL

BACKGROUND: Associations between acne and gastrointestinal comorbidities suggest that microbial dysbiosis and intestinal permeability may promote inflammatory acne, a condition often managed with oral antibiotics. OBJECTIVE: We performed a case-control study...
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Evaluation of fecal microbiomes associated with obesity in captive cynomolgus monkeys (Macaca fascicularis)

Koo BS, Hwang EH, Kim G, Oh H, Son Y, Lee D, Lim KS, Kang P, Lee S, Lee HY, Jeong KJ, Lee Y, Baek SH, Jeon CY, Park SJ, Kim YH, Huh JW, Jin YB, Kim SU, Lee SR, Hong JJ

Microorganisms play important roles in obesity; however, the role of the gut microbiomes in obesity is controversial because of the inconsistent findings. This study investigated the gut microbiome communities in...
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Concomitant Liver and Brain Abscesses Caused by Parvimonas Micra

Kim EY, Baek YH, Jung DS, Woo KS

Anaerobic infections have been reported to be responsible for 3–10% of pyogenic liver abscesses in Korea, and reported anaerobes include Fusobacterium, Bacillus fragilis, and Bacteroides melaninogenicus. Parvimonas micra is an...
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Microbiome diversity in carriers of fluoroquinolone resistant Escherichia coli

Liss MA, Leach RJ, Rourke E, Sherrill A, Johnson-Pais T, Lai Z, Basler J, White JR, Patterson

PURPOSE: Fluoroquinolone-resistant (FQR) Escherichia coli causes transrectal prostate biopsy infections. In order to reduce colonization of these bacteria in carriers, we would like to understand the surrounding microbiome to determine...
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Antimicrobial Susceptibility Patterns of Anaerobic Bacterial Clinical Isolates From 2014 to 2016, Including Recently Named or Renamed Species

Byun JH, Kim M, Lee Y, Lee K, Chong Y

BACKGROUND: Anaerobic bacterial resistance trends may vary across regions or institutions. Regional susceptibility patterns are pivotal in the empirical treatment of anaerobic infections. We determined the antimicrobial resistance patterns of...
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Relationship of Oral Bacterial Load Over One Year of Smoking Cessation

Kim S, Seo MS, Hwang SJ

BACKGROUND: Smoking exerts an adverse effect on the periodontal tissue by reorganizing the ecosystem of oral microorganisms and is considered to be an important factor in the development of periodontal...
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Analysis of Intestinal Mucosal Microbiome Changes before and after Chemoradiation in Locally Advanced Rectal Cancer Patients

Seo I, Bae SU, Kim S, Jeong WK, Baek SK

PURPOSE: Dysbiosis of gut microbiota has been reported to participate in the pathogenesis of colorectal cancer, but changes in microbiota due to radiotherapy have not been studied. In this study,...
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Delayed intraorbital infection after craniofacial bone surgery

Jung JS, Lim NK, Kang DH

Intraorbital infection shows a low incidence, but it might cause blindness or even death. This case is unusual in that its origin from a craniofacial bone fracture prior to infection...
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Real-time PCR quantification of 9 periodontal pathogens in saliva samples from periodontally healthy Korean young adults

Choi H, Kim E, Kang J, Kim HJ, Lee JY, Choi J, Joo JY

PURPOSE: Few studies have examined periodontal pathogens from saliva samples in periodontally healthy young adults. The purposes of this study were to determine the prevalence of periodontopathic bacteria and to...
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Utility of Conventional Culture and MALDI-TOF MS for Identification of Microbial Communities in Bronchoalveolar Lavage Fluid in Comparison with the GS Junior Next Generation Sequencing System

Sung JY, Hwang Y, Shin MH, Park MS, Lee SH, Yong D, Lee K

BACKGROUND: Diverse microbiota exist in the lower respiratory tract. Although next generation sequencing (NGS) is the most widely used microbiome analysis technique, it is difficult to implement NGS in clinical...
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An Advanced Understanding of Uterine Microbial Ecology Associated with Metritis in Dairy Cows

Jeon SJ, Galvão KN

Metritis, the inflammation of the uterus caused by polymicrobial infections, is a prevalent and costly disease to the dairy industry as it decreases milk yield, survival, and the welfare of...
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Differences Regarding the Molecular Features and Gut Microbiota Between Right and Left Colon Cancer

Kim K, Castro EJ, Shim H, Advincula , Kim YW

For many years, developmental and physiological differences have been known to exist between anatomic segments of the colorectum. Because of different outcomes, prognoses, and clinical responses to chemotherapy, the distinction...
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Acute Delayed or Late Infection of Revision Total Hip Arthroplasty Treated with Debridement/Antibiotic-loaded Cement Beads and Retention of the Prosthesis

Chang JD, Kim IS, Lee SS, Yoo JH

PURPOSE: The treatment of infected revision total hip arthroplasty (THA) is very challenging due to retained revision prosthesis, poor bone stock and soft tissue condition derived from previous revision surgeries,...
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Red fluorescence of oral bacteria is affected by blood in the growth medium

Jeong SH, Yang YH, Lee MA, Kim SY, Kim JS

OBJECTIVES: Dental plaque emits red fluorescence under a visible blue light near the ultra-violet end of the light spectrum. The fluorescence characteristics of each microorganism have been reported in several...
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Antimicrobial Effect of Coptidis rhizome Extract against Mutans Streptococci and Periodontopathogens

Park SN, Lim YK, Kook JK

The purpose of the study was to investigate the antimicrobial activity of the methanol extract of Coptidis rhizome against the type strains of cariogenic bacteria, Streptococcus mutans and Streptococcus sobrinus,...
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Polymicrobial Purulent Pericarditis Probably caused by a Broncho-Lymph Node-Pericardial Fistula in a Patient with Tuberculous Lymphadenitis

Lee S, Lee K, Ko JK, Park J, Yu MY, Oh CK, Hong SP, Kim Y, Lim Y, Kim H, Pai H

Purulent pericarditis is a rare condition with a high mortality rate. We report a case of purulent pericarditis subsequently caused by Candida parapsilosis, Peptostreptococcus asaccharolyticus, Streptococcus anginosus, Staphylococcus aureus, Prevotella...
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Detection of Bacterial Species in Chronic Periodontitis Tissues at Different Stages of Disease Severity

Yoon DL, Kim S, Song H, Kim YG, Lee JM, Kim J

The goal of this research was to determine the relationship between the stage of chronic periodontitis and the presence of six bacterial pathogens (Aggregatibacter actinomycetemcomitans: AA, Fusobacterium nucleatum: FN, Porphyromonas...
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Pilonidal Abscess Associated With Primary Actinomycosis

Oh HB, Abdul Malik MH, Keh CH

Pilonidal disease in the sacrococcygeal region usually presents as abscesses, recurrent inflammation, cellulitis or fistula tracks. However, few reports on actinomycosis affecting pilonidal sinuses have been published. We report a...
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Development of Quantitative Real-Time PCR Primers for Detection of Prevotella intermedia

Park SN, Kook JK

Prevotella intermedia-specific quantitative real-time PCR (qPCR) primers were previously designed based on the nucleotide sequences of RNA polymerase beta-subunit gene (rpoB). However, the several clinical strains isolated from Korean populations...
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