J Korean Med Sci.  1993 Aug;8(4):293-304. 10.3346/jkms.1993.8.4.293.

Immunohistochemical detection of p53 protein, c-erbB-2 protein, epidermal growth factor receptor protein and proliferating cell nuclear antigen in gastric carcinoma

• Jang WI
• Yang WI
• Lee CI
• Kim HS
• Song KS
• Cho MY
• Park JK
• Shim YH

• KMID: 2157514
• DOI: http://doi.org/10.3346/jkms.1993.8.4.293

Abstract

There is increasing evidence that genes involved in normal cell growth and differentiation (oncogenes) or genes that encode for growth factors are important in determining the development and biologic aggressiveness of gastric carcinoma. This study was undertaken to define the prognostic value of the overexpression of p53 protein, c-erbB-2 protein, EGFr protein and PCNA in gastric carcinomas. Using monoclonal antibodies, immunohistochemical studies were performed on formalin-fixed, paraffin-embedded tissue sections from 84 primary gastric carcinomas. Overall, 34% of gastric carcinomas had nuclear-staining for p53 protein, 34% of carcinomas membrane staining for the c-erbB-2 and 74% of carcinomas membrane and cytoplasmic staining for EGFr, showing distribution in a heterogeneous fashion. PCNA was expressed as Grade 2 and 3 in 75% of patients with gastric carcinomas. Both c-erbB-2 and p53 staining was significantly associated with high grade expression of PCNA. p53 staining tended to be associated with positive nodal status and metastasis, and c-erbB-2 staining with positive nodal status only. Multivariate analysis using the Cox model showed that overexpression of p53 protein, c-erbB-2 protein and PCNA was not an independent prognostic variable in gastric carcinoma. These results suggest that expressions of p53 and c-erbB-2 protein are heterogeneous and that p53 and c-erbB-2 overexpressions are significantly associated with high proliferative activity in gastric carcinoma.

Keyword

p53; c-erbB-2; Epidermal growth factor receptor; Proliferating cell nuclear antigen; Gastric carcinoma

MeSH Terms

Antigens, Neoplasm/analysis
Humans
Immunohistochemistry
Multivariate Analysis
Neoplasm Proteins/analysis
Nuclear Proteins/*analysis
Prognosis
Proliferating Cell Nuclear Antigen
Proto-Oncogene Proteins/*analysis
Receptor, Epidermal Growth Factor/*analysis
Receptor, erbB-2
Retrospective Studies
Stomach Neoplasms/*chemistry/immunology/mortality
Survival Rate
Tumor Suppressor Protein p53/*analysis
Antigens, Neoplasm
Neoplasm Proteins
Nuclear Proteins
Proliferating Cell Nuclear Antigen
Proto-Oncogene Proteins
Tumor Suppressor Protein p53
Receptor, Epidermal Growth Factor
Receptor, erbB-2