J Rhinol.  2002 Nov;9(1, 2):52-56.

Effect of Macrolides on the Interleukin-1beta-mediated MUC2/5AC Genes Expression and Mucin Secretion in Human Airway Epithelial Cells

Affiliations
  • 1Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University, Daegu, Korea. ydkim@med.yu.ac.kr

Abstract

BACKGROUND AND OBJECTIVES: Macrolide is a relatively effective drug in chronic bronchiolitis and chronic sinusitis with mucous hypersecretion. However, the anti-secretory effect of macrolide is not clear. The aim of this study was to evaluate the effect of macrolide on the interleukin -1beta (IL-1beta)-induced MUC2/5AC genes expression and mucin secretion. Matrials and Methods: We observed effects of roxithromycin and clarithromycin on the IL-1beta-induced MUC2/5AC genes expression and mucin secretion in cultured human airway NCI-H292 epithelial cells. The steady state mRNA levels of MUC2/5AC and mucin secretion were determined by reverse transcriptase-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay.
RESULTS
Roxithromycin attenuated the IL-1beta-mediated MUC2/5AC genes expression and mucin secretion. When roxithromycin treated before exposure to IL-1beta and after exposure to IL-1beta in cultured cells, both treatment methods inhibited the IL-1beta-induced MUC2/5AC genes expression and mucin secretion. However, clarithromycin did not suppress the IL-1beta-mediated MUC2/5AC genes expression and mucin secretion.
CONCLUSION
This result suggests that roxithromycin inhibits the IL-1beta-mediated mucin secretion through inhibition of MUC2/5AC genes expression.

Keyword

MUC2; MUC5AC; Mucin; Roxithromycin; Clarithromycin
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