Korean J Otolaryngol-Head Neck Surg.  2004 Nov;47(11):1115-1119.

MUC2/5AC Expression and Mucin Secretion through Leukotriene Receptor in Human Airway Epithelial Cells

• Kim YD
• Lee JE
• Bai CH
• Seo YJ
• Ye SB
• Song SY

Abstract

BACKGROUND AND OBJECTIVES
Mucin gene expression and mucin production are highly increased during inflammatory airway disorders such as, asthma, chronic bronchitis and sinusitis. Cytokines, lipopolysaccharides and other inflammatory mediators are related with secretion and production of mucin. However, among of inflammatory mediators, the relation of leukotrienes and mucin genes expression is not clear. The aim of this study is to evaluate MUC2/5AC genes expression and mucin secretion through leukotriene receptor in human airway epithelial cells. SUBJECTS AND METHOD: The effect of Leukotriene D4 and leukotriene receptor antagonist, pranlukast hydrate (ONO-1078) on the regulation of MUC2/5AC gene expression and mucin secretion was observed in the human airway NCI-H292 epithelial cells. The mRNA levels of MUC2/5AC and the amount of mucin protein were determined by reverse transcription-polymerase chain reaction (RT-PCR) and immunoassay. RESULTS: Leukotriene D4 upregulated MUC2/5AC gene expression and mucin secretion on a dose dependent pattern. Pranlukast hydrate (ONO-1078, 100 micrometer) downregulated the leukotriene D4-mediated MUC2/5AC gene expression and mucin secretion. CONCLUSION: These results suggest that the leukotriene receptor system is one of the expression mechanisms of MUC2/5AC genes and mucin secretion.

Keyword

Leukotriene D4; Leukotriene receptor antagonists; MUC2; MUC5AC; Mucin

MeSH Terms

Asthma
Bronchitis, Chronic
Cytokines
Epithelial Cells*
Gene Expression
Humans*
Immunoassay
Leukotriene Antagonists
Leukotriene D4
Leukotrienes
Lipopolysaccharides
Mucins*
Receptors, Leukotriene*
RNA, Messenger
Sinusitis
Cytokines
Leukotriene Antagonists
Leukotriene D4
Leukotrienes
Lipopolysaccharides
Mucins
RNA, Messenger
Receptors, Leukotriene