Korean J Otolaryngol-Head Neck Surg.  2004 Jul;47(7):639-644.

Vasoactive Intestinal Peptide Induces MUC2/5AC Synthesis in Human Airway Epithelial Cells

• Kim YD
• Chang KY
• Sin JH
• Kwak DS
• Lee HJ
• Song SY
• Song KW

Abstract

BACKGROUND AND OBJECTIVES
Vasointestinal peptide (VIP) is an important neurotransmitter involved in the regulation of mucus secretion, but the relationship of VIP and mucin genes is not clear. This study was designed to investigate the effect of VIP on MUC2/5AC genes expression and mucin secretion in human airway epithelial cells. MATERIALS AND METHOD: The mRNA levels of MUC2/5AC genes and mucin secretion were determined by RT-PCR and the immunoblot method in cultured human airway NCI-H292 epithelial cells. RESULTS: VIP (10-6-10-10 M) induced MUC2/5AC gene expression and mucin secretion in a reverse dose-dependant manner. The maximum expression of mRNA and mucin secretion level of MUC2/5AC was 10-10 M of VIP. Actinomycin D inhibited the VIP-mediated MUC2/5AC gene expression and mucin secretion, but cycloheximide did not. Budesonide attenuated the VIP-mediated MUC2/5AC genes expression and mucin secretion. RU-486, a glucocorticoid receptor antagonist, restored the inhibitory effect of budesonide. CONCLUSION: These results suggest that VIP regulates MUC2/5AC gene expression and secret mucin by transcriptional regulation, and that budesonide inhibits the VIP-mediated MUC2/5AC genes expression and mucin secretion through the glucocorticoid receptor.

Keyword

Vasoactive intestinal peptide; MUC2; MUC5AC; Mucin; Budesonide

MeSH Terms

Budesonide
Cycloheximide
Dactinomycin
Epithelial Cells*
Gene Expression
Humans*
Mifepristone
Mucins
Mucus
Neurotransmitter Agents
Receptors, Glucocorticoid
RNA, Messenger
Vasoactive Intestinal Peptide*
Budesonide
Cycloheximide
Dactinomycin
Mifepristone
Mucins
Neurotransmitter Agents
RNA, Messenger
Receptors, Glucocorticoid
Vasoactive Intestinal Peptide