J Korean Ophthalmol Soc.
2001 Jun;42(6):852-856.
Cell Death of Corneal Fibroblasts Induced by Tumor Necrosis Factor-alphaand Interferon-gamma
- Affiliations
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- 1Department of Ophthalmology, Samsung Medical Center, Sungkyunkwan University, School of Medicine, Seoul, Korea.
- 2Department of Ophthalmology, Kangwon National University College of Medicine, Chunchon, Korea.
Abstract
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PURPOSE: To evaluate the effect in the cell death of corneal fibroblasts when TNF-alphaand INF-gamma were given together.
METHODS
Fibroblasts harvested from the human cornea were cultured in DMEM, then, nothing(control: Group 1), TNF-alphaonly(50 ng/ml : Group 2), INF-gammaonly(1.0 x 10(3)u/ml : Group 3), and a combination of both(Group 4) were added. We assessed the cell viability of the each group by the trypan blue exclusion assay at 4, 8, 12, 24, 48 hours after addition of cytokines.
RESULTS
The cell viability at 48 hour after treatment was 94.27% in group 1, 90.68%(p=0.09) in group 2, 93.31%(p=0.45) in group3, and there was no statistical difference among the groups. Statistically signi-ficant decrease of the cell viability was achieved in group 4(82.86%, p=0.002).
CONCLUSIONS
Cell death of human corneal fibroblasts had been increased after treatment with a combination of TNF-alphaand INF-gamma. These findings suggest that there could be some kind of interaction among the cytokines.
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