J Bacteriol Virol.
2002 Dec;32(4):393-400.
Depression of Both Interferon-gamma and Tumor Necrosis Factor-alpha Production by Peripheral Blood Mononuclear Cells from Chronic Refractory Tuberculosis Patients
- Affiliations
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- 1Department of Microbiology, College of Medicine, Chungnam National University, 6 Munhwa-dong, Jung-ku, Daejeon, Korea. hayoungj@cnu.ac.kr
- 2Department of Microbiology, College of Medicine, Konyang University, Nae-dong, Nonsan, Chungnam, Korea.
- 3National Mokpo Tuberculosis Hospital, 45 Seokhyun-dong, Mokpo, Korea.
Abstract
- Understanding human immune responses in chronic refractory tuberculosis (CRTB) is important for developing immunotherapy against the disease. The aim of this study was to examine cytokine responses [interferon (IFN)-gamma, tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, and IL-10] by peripheral blood mononuclear cells (PBMCs) in CRTB patients after in vitro stimulation with the 30-kDa or purified protein derivative (PPD) antigen (Ag). Most of the CRTB cases were multidrug-resistant (MDR) TB. The results were compared with those from early TB (E-TB) patients and healthy tuberculin reactors (HTR). IFN-gamma production was significantly depressed in both CRTB and E-TB groups compared with HTR. In response to the 30-kDa Ag, TNF-alpha levels were significantly depressed only in CRTB patients, while greatly increased in E-TB patients. In addition, IL-10 production was significantly increased in E-TB patients, and PBMC from both E-TB and CRTB patients secreted more IL-6 than HTR. IL-10 neutralization significantly increased TNF-alpha levels, whereas anti-TNF-alpha did not alter IL-10 induction significantly in PBMC from HTR and CRTB patients. Our findings suggest that CRTB patients have depression in both IFN-gamma and TNF-alpha reponses, which might play important roles during chronic M. tuberculosis infection.
Keyword
MeSH Terms
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Depression*
Humans
Immunotherapy
Interferon-gamma*
Interleukin-10
Interleukin-12
Interleukin-6
Interleukins
Mycobacterium tuberculosis
Tuberculin
Tuberculosis*
Tuberculosis, Multidrug-Resistant
Tumor Necrosis Factor-alpha*
Interferon-gamma
Interleukin-10
Interleukin-12
Interleukin-6
Interleukins
Tuberculin
Tumor Necrosis Factor-alpha
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