J Korean Assoc Maxillofac Plast Reconstr Surg.  2013 May;35(3):143-154.

Induction of Selective Cell Death of Oral Squamous Carcinoma Cells by Integrin alpha2 Antibody and EGFR Antibody

Affiliations
  • 1Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Korea. kuksjs@pusan.ac.kr
  • 2Department of Oral Anatomy, School of Dentistry, Pusan National University, Korea.
  • 3Department of Anatomy, School of Medicine, Pusan National University, Korea.
  • 4Department of Oral and Maxillofacial Surgery, College of Medicine, Dong-A University, Korea.
  • 5Department of Prothodontics, School of Dentistry, Pusan National University, Korea.
  • 6Department of Oral and Maxillofacial Surgery, School of Medicine, Gyeongsang National University, Korea.

Abstract

PURPOSE
This study was to find efficacy of integrin alpha2 (alpha2) and epidermal growth factor receptor (EGFR) as tumor marker of oral squamous cell carcinoma (SCC) and clarify the selective cell death effect of anti-integrin alpha2 and anti-EGFR on SCC cells, additionally testify conjugated gold nanoparticles (GNP) with air plasma for selective cell death of oral SCC.
METHODS
Expression of integrin alpha2, EGFR on human SCC cells (SCC25) were examined by western blot. SCC25 cells were treated with anti-integrin alpha2, anti-EGFR and analysed by Hemacolor staining, immunoflorescence staining, FACS flow cytometry. Conjugated GNP with integrin alpha2, EGFR antibody were treated by air plasma on SCC cells.
RESULTS
Integrin alpha2 and EGFR were over-expressed on SCC25 cells than normal lung WI-38 cells. The cell viability rate of SCC25 cells treated with anti-integrin alpha2, anti-EGFR was lower than WI-38 cells. The concentration changes of nucleus, releasing cytochrome c and apoptosis inducing factor (AIF) from mitochondria to cytosol were observed. The changes of proteins related with apoptosis were observed. Increase of bax, bcl-xL, activation of caspase-3, -7, -9, and fragmentation of PARP, DFF45 and decrease of lamin A/C in SCC25 cells were observed. In FACS, increase of sub-G1 and S phase was observed. Cell cycle related proteins, Such as cyclin D1, cyclin dependent kinase (CDK) 4, cyclin A, cyclin E, CDK 2, p27 were decreased. After SCC25 cells treated with conjugatged GNP-Integrin alpha2, GNP-EGFR, additionally air plasma, the cell death rate was significantly increased.
CONCLUSION
Integrin alpha2, EGFR were over-expressed in oral SCC cells. Anti-integrin alpha2, anti-EGFR in SCC25 cells induced apoptosis selectively. When GNP-anti integrin alpha2, GNP-anti EGFR were treated with air plasma on SCC25 cells, cancer cells were died more selectively. GNP-anti integrin alpha2, GNP-anti EGFR with air plasma could be treatment choice of oral SCC.

Keyword

OSCC; Integrin alpha2; EGFR; Gold nanoparticles; Cell death

MeSH Terms

Apoptosis
Apoptosis Inducing Factor
Blotting, Western
Carcinoma, Squamous Cell
Caspase 3
Cell Cycle
Cell Death
Cell Survival
Cyclin A
Cyclin D1
Cyclin E
Cyclins
Cytochromes c
Cytosol
Flow Cytometry
Humans
Integrin alpha2
Lung
Mitochondria
Nanoparticles
Phosphotransferases
Plasma
Proteins
Receptor, Epidermal Growth Factor
S Phase
Apoptosis Inducing Factor
Caspase 3
Cyclin A
Cyclin D1
Cyclin E
Cyclins
Cytochromes c
Integrin alpha2
Phosphotransferases
Proteins
Receptor, Epidermal Growth Factor
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