J Korean Neurol Assoc.  1992 Sep;10(3):255-267.

The Effect of Nimodipine on Size of Infarction and the Regional Cerebral Blood Flow in Focal Cerebral Ischemia of Rats

• Lim BH
• Roh JK
• Hong SB
• Yoon BW
• Lee SB
• Lee MC
• Kim SE

Abstract

To evaluate the effect of nimodipine on the size of infarction and regional cerebral blood flow(rCBF), focal cerebral ischemia was produced by middle cerebral artery occlusion(MCAO) in Sprague-Dawley rats. The experimental groups were divided into a saline-treated(control) group and a nimodipine-treated group. The rats in the nimodipine-treated group were intravenously loaded with 10 ug/kg of nimodipine 30 min before and the rats in the control group of 1 ug/kg/min over next 1 hour by an infusion pump and the rats in the control group were given the same volume of saline by the same method. The size of infarction were measured by staining the coronal sections of the brain with 2% 2,3,5-triphenyltetrazolium chloride(TTC) solution and the regional cerebral blood flows(rCBFs) were calculated by an autoradiography using 14C-iodoantipyrine. The results were summarized as follows : 1. Arterial blood pH, PCO2, PO2 and blood glucose level determination were done just after MCAO. There were no significant differences between the two groups 2. Arterial blood pressure was monitored from 30 minutes after MCAO every 5 min. There were no significant changes of blood pressure in both groups. 3. The size of infarction were measured and compared between the two groups by TTC staining. The sizes of infarction were siginificantly decreased in the nimodipine-treated group(P < 0.05) 4. Regional cerebral blood flows in the nimodipine-treated group were significantly improved in the areas neighboring to the dense ischemic core, somatosensory area of frontal and parietal lobes. The areas which showed a significant improvement in the rCBF were the motor areas of frontal and parietal cortices, caudate head, anterior caudoputamen, amygdala, CA3 area and auditory cortex. 5. The serial measurement of rCBF of cerebral cortex with 1 mm's interval showed a significant improvement of rCBF in the areas neighboring to the ischemic core in the nimodipine-treated group(P < 0.05) 6. The area with the rCBF under 25 ml/100g/min was significantly decr.

MeSH Terms

Amygdala
Animals
Arterial Pressure
Auditory Cortex
Autoradiography
Blood Glucose
Blood Pressure
Brain
Brain Ischemia*
Cerebral Cortex
Head
Hydrogen-Ion Concentration
Infarction*
Infusion Pumps
Middle Cerebral Artery
Nimodipine*
Parietal Lobe
Rabeprazole
Rats*
Rats, Sprague-Dawley
Blood Glucose
Nimodipine