J Korean Med Sci.  2004 Jun;19(3):321-326. 10.3346/jkms.2004.19.3.321.

Effects of HMGB-1 Overexpression on Cell-Cycle Progression in MCF-7 Cells

• Yoon S
• Lee JY
• Yoon BK
• Bae DS
• Choi DS

• KMID: 1786806
• DOI: http://doi.org/10.3346/jkms.2004.19.3.321

Abstract

High mobility group-1 (HMGB-1) enhances the DNA interactions and possesses a transcriptional activation potential for several families of sequence-specific transcriptional activators. In order to examine the effect of HMGB-1 on the cell cycle progression in MCF-7 cells, the HMGB-1 expression vector was transfected into synchronized MCF-7 cells, and the effect of HMGB-1 overexpression on the cell cycle was examined. The HMGB-1 protein level in the transfected cells increased 4.87-fold compared to the non-transfected cells. There were few changes in the cell cycle phase distribution after HMGB-1 overexpression in the MCF-7 cells. Following the estrogen treatment, the cell cycle progressed in both the HMGB-1 overexpressed MCF-7 and the mock-treated cells. However, a larger proportion of HMGB-1 overexpressing MCF-7 cells progressed to the either S or G2 phase than the mock-treated cells. The mRNA levels of the cell cycle regulators changed after being treated with estrogen in both the HMGB-1 overexpressing MCF-7 and the mock-treated cells, but the changes in the expression level of the cell cycle regulator genes were more prominent in the HMGB-1 overexpressing MCF-7 cells than in the mock-treated cells. In conclusion, HMGB-1 overexpression itself does not alter the MCF-7 cell cycle progression, but the addition of estrogen to the HMGB-1 overexpressing MCF-7 cells appears to accelerate the cell cycle progression.

Keyword

Cell Cycle; HMG-Box Domains; HMGB1 Protein; Receptors, Estrogen

MeSH Terms

Blotting, Western
Cell Cycle
Cell Line, Tumor
Densitometry
Estrogens/metabolism
G2 Phase
Genetic Vectors
HMGB1 Protein/*biosynthesis
Human
Kinetics
Oligonucleotides/chemistry
Plasmids/metabolism
Protein Structure, Tertiary
RNA, Messenger/metabolism
Reverse Transcriptase Polymerase Chain Reaction
S Phase
Support, Non-U.S. Gov't
Time Factors
Trans-Activation (Genetics)
Transfection