J Breast Cancer.  2012 Dec;15(4):388-392. 10.4048/jbc.2012.15.4.388.

Effect of bFGF on the MCF-7 Cell Cycle with CD44+/CD24-: Promoting the G0/G1-->G2/S Transition

• Yang ZL
• Cheng K
• Han ZD

• KMID: 2286434
• DOI: http://doi.org/10.4048/jbc.2012.15.4.388

Abstract

PURPOSE
Few cells with stem cell characteristics possess capabilities of self-renewal and differentiation, which leads to high tumorigenesis and resistance to standard chemotherapeutic agents. These cells are mostly quiescent, and arrest occurs at the mitotic G0/G1 phase in mitosis. We explored the effects of basic fibroblast growth factor (bFGF) on the MCF-7 cell cycle with CD44+/CD24-.
METHODS
Cancer-initiating cells were propagated as mammospheres. The CD44+/CD24- subpopulation was sorted by a fluorescence activating cell sorter-Vantage flow cytometer. A cell cycle analysis was performed with different bFGF concentrations.
RESULTS
Differences in the CD44+/CD24- cell proliferation under different bFGF concentrations were observed (p=0.001). When the bFGF concentration was increased, the proportion of CD44+/CD24- at G0/G1 decreased (p=0.023).
CONCLUSION
We conclude that bFGF may sustain CD44+/CD24- cell proliferation and could promote cell progression through the G0/G1-->G2/S phase transition.

Keyword

Breast neoplasms; Cell cycle; Fibroblast growth factors; Stem cells

MeSH Terms

Breast Neoplasms
Cell Cycle
Cell Proliferation
Cell Transformation, Neoplastic
Fibroblast Growth Factor 2
Fibroblast Growth Factors
Fluorescence
MCF-7 Cells
Mitosis
Phase Transition
Stem Cells
Fibroblast Growth Factor 2
Fibroblast Growth Factors