- Comparison of Various Transfection Methods in Human and Bovine Cultured Cells
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Jin L, Kim D, Roh S
- KMID: 2170785
- Int J Oral Biol.
- 2014 Dec;39(4):177-185.
- doi: 10.11620/IJOB.2014.39.4.177
Transfection is a gene delivery tool that is a popular means of manipulating cellular properties, such as induced pluripotent stem cell (iPSC) generation by reprogramming factors (Yamanaka factors). However, the... -
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- Anticancer Activity of RetinoblastomaRB Gene Transfection in Cultured Ovarian Cancer Cells
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Park SY, Park YG, Lee GW, Lee YH, Kim YT, Kang JS, Lee GI, Koo BS
- KMID: 2036195
- Korean J Obstet Gynecol.
- 2000 Dec;43(12):2178-2184.
No abstract available. -
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- Improved Transfection Efficiency and Metabolic Activity in Human Embryonic Stem Cell Using Non-Enzymatic Method
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Kim CY, Hwang IK, Kang C, Chung EB, Jung CR, Oh H, Jeong YH, Moon SH, Kim JS, Hong KS, Park JH, Chung HM
- KMID: 2429547
- Int J Stem Cells.
- 2018 Nov;11(2):149-156.
- doi: 10.15283/ijsc18037
Human embryonic stem cells (hESCs) are pluripotent cells widely used in conventional and regenerative medicine due to their ability to self-renew, proliferate and differentiate. Recently, genetic modification of stem cells... -
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- Enhanced Expressions and Histological Characteristics of Intravenously Administered Plasmid DNA in Rat Lung
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Rha SJ, Wang YP
- KMID: 2157563
- J Korean Med Sci.
- 2001 Oct;16(5):567-572.
- doi: 10.3346/jkms.2001.16.5.567
Cationic liposome-mediated gene transfection is a promising method for gene therapy. In this study, the transfection efficiency and histological patterns were evaluated in rat lung after intravenous administration via femoral... -
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- The Effect of Transfection of RET9 and RET51 on Apoptosis in SW48 and RKO Colon Cancer Cells
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Seo HI, Park DI
- KMID: 2054658
- Intest Res.
- 2013 Jan;11(1):28-33.
- doi: 10.5217/ir.2013.11.1.28
BACKGROUND/AIMS: Dependent receptor can transmit both positive signal: proliferation, differentiation or migration; and negative signal: apoptosis. It depends on the presence of its ligand. This study was performed to determine... -
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- Development of Gene Therapy Strategy Using Plasmid and Adenovirus in Cervical Cancer Treatment
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Lee JM, Kim SJ, NamKoong S, Cho SD, Hwang SJ, Park HR, Han YJ, Kim ST, Lee HY, Kim DJ, Park YS, Kim CK, Oh YK, Park SH, Ahn WS
- KMID: 2269870
- Korean J Obstet Gynecol.
- 1999 Sep;42(9):2019-2027.
BACKGROUND: The basic treatment of malignant tumors is surgery, radiotherapy, chemotherapy. Even though, the object of these treatments is to kill cancer cells, they have limitations. So, in future studies... -
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- Comparison of Ectopic Gene Expression Methods in Rat Neural Stem Cells
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Kim W, Kim JH, Kong SY, Park MH, Sohn UD, Kim HJ
- KMID: 1432759
- Korean J Physiol Pharmacol.
- 2013 Feb;17(1):23-30.
- doi: 10.4196/kjpp.2013.17.1.23
Neural stem cells (NSCs) have the ability to proliferate and differentiate into various types of cells that compose the nervous system. To study functions of genes in stem cell biology,... -
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- Increased Viability by Over-Expression of Bcl-XL in a Human Neural Progenitor Cell Line
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Ahn YH, Shin YS, Yoon SH, Cho KH, Cho KG, Kim SU
- KMID: 2188953
- J Korean Neurosurg Soc.
- 2004 Apr;35(4):415-421.
OBJECTIVE: This study is aimed whether bcl-xl could protect C4 cells from the cell death induced by serum deprivation. METHODS: The transient transfection of the bcl-xl gene was made with... -
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- Changes of Transfection Efficiencies of Plasmid LacZ Genes in Human Gynecologic Cancer Cell Lines
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Kim BH, Bae SM, Han YJ, Lee HS, Jo YS, Lee KH, Hur SY, Kim JH, Kim YW, Ro DY, Lee JM, Namkoong S, Kim DJ, Kim CK, Ahn WS
- KMID: 2272332
- Korean J Obstet Gynecol.
- 2004 May;47(5):870-879.
OBJECTIVE: The transfection efficiencies of gynecologic cancer cell lines were investigated by different mediated transfection methods using recombinant LacZ plasmid (pRcCMVLacZ and pAAVCMVLacZ). METHODS: In this study, the gynecologic cancer cell... -
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- The Effect of Induced Heat Shock Protein 33 in Human Corneal Epithelial Cell
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Han JS, Ko SM, Lee HG, Kim JC
- KMID: 2125507
- J Korean Ophthalmol Soc.
- 2003 Oct;44(10):2353-2357.
PURPOSE: The purposes of study was to assess the expression patterns of heat shock protein 33 (HSP33) after gene transfection and to evaluate the protective effects of heat shock protein... -
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- RET: A Multi-Faceted Gene in Human Cancer
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Santoro M, Carlomagno F, Melillo RM
- KMID: 2282399
- Endocrinol Metab.
- 2012 Sep;27(3):173-179.
- doi: 10.3803/EnM.2012.27.3.173
REarranged during Transfection (RET) gene encodes a receptor tyrosine kinase and it was initially discovered as an in vitro transforming gene. For many years, RET has been involved in papillary... -
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- Sendai F/HN Viroplexes for Efficient Transfection of Leukemic T Cells
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Kim JS, Lee YK, Jeong HY, Kang SJ, Kim MW, Ryu SH, Kim HS, Kim KS, Kim DE, Park YS
- KMID: 1793162
- Yonsei Med J.
- 2013 Sep;54(5):1149-1157.
- doi: 10.3349/ymj.2013.54.5.1149
PURPOSE: Most chemical transfection reagents are ineffective for the transfection of cells in suspension, such as leukemic cell and stem cell lineages. We developed two different types of viroplexes, cationic... -
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- Transfection of SV 40 Large T Antigen into Corneal Endothelial Cells
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Cho KS, Fleming TP, Lee EH, Joo CK
- KMID: 2205471
- J Korean Ophthalmol Soc.
- 1999 Mar;40(3):676-682.
The coeneal endothelium is essential for the maintenance of normal corneal hydration, thickness, and transparency. However, corneal endothelial cells are incapable of significant proliferation in vivo. As we age, the... -
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- Cell Death Mechanisms in Esophageal Squamous Cell Carcinoma Induced by Vesicular Stomatitis Virus Matrix Protein
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Douzandegan Y, Tahamtan A, Gray Z, Nikoo HR, Tabarraei , Moradi A
- KMID: 2456577
- Osong Public Health Res Perspect.
- 2019 Aug;10(4):246-252.
- doi: 10.24171/j.phrp.2019.10.4.08
OBJECTIVES: Vesicular stomatitis virus (VSV) is under development as an oncolytic virus due to its preferential replication in cancer cells and oncolytic activity, however the viral components responsible have not... -
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- Monitoring transfected cells without selection agents by using the dual-cassette expression EGFP vectors
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Kang SG, Lee EK, Schaus S, Henderson
- KMID: 938883
- Exp Mol Med.
- 2001 Sep;33(3):174-178.
Conventional methods of selecting gene transfected cells by toxic agents may yield ambiguous results. It is difficult to determine whether cell death is due to selection agents or gene transfection,... -
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- Clusterin confers paclitaxel resistance in ovarian cancer
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Park DC, Yeo SG, Mok SC
- KMID: 2272700
- Korean J Obstet Gynecol.
- 2005 Oct;48(10):2313-2320.
OBJECTIVE: To evaluated whether clusterin over-expression is significantly correlated with paclitaxel resistance in ovarian cancer cell lines. METHODS: Clusterin was validated by performing expression profiling analysis and subsequently, the correlation between... -
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- Effects of Wild - type p53 Gene Transfection into Human Colon Cancer Cell Line
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Kim HO, Kim WH, Bae SI, Lee HW, Kim CJ, Hong SY, Kim YI
- KMID: 2175234
- J Korean Cancer Assoc.
- 1999 Apr;31(2):367-376.
PURPOSE: In colon cancer, the most frequent genetic alteration is found in p53 tumor suppressor gene residing on the short arm of chromosome 17. In order to investigate the significance... -
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- The optimal concentration of siRNA for gene silencing in primary cultured astrocytes and microglial cells of rats
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Ki KH, Park DY, Lee SH, Kim NY, Choi BM, Noh GJ
- KMID: 1766501
- Korean J Anesthesiol.
- 2010 Dec;59(6):403-410.
- doi: 10.4097/kjae.2010.59.6.403
BACKGROUND: Small interfering RNAs (siRNAs) have been used to knockdown specific gene expression in various cells. Astrocytes and microglial cells play a key role in fundamental central nervous system functions... -
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- Inductoin of Radioresistance by Overexpression of Glutathione S-Transferase K1 (hGSTK1) in MCF-7 Cells
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Kim JC, Shin SO
- KMID: 1613505
- J Korean Soc Ther Radiol Oncol.
- 2001 Dec;19(4):381-388.
PURPOSE: This study was conducted to assess the effects of x-irradiation on the expression of the novel glutathione S-transferase K1 gene. MATERIALS AND METHODS: Human glutathione S-transferase K1 (hGSTK1) DNA... -
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- The Combined Effect of Gamma Knife Irradiation and p53 Gene Transfection in Human Malignant Glioma Cell Lines
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Kim JE, Paek SH, Kim DG, Chung HT, Kim YY, Jung HW
- KMID: 1817828
- J Korean Neurosurg Soc.
- 2005 Jan;37(1):48-53.
OBJECTIVE: The purpose of this study is to elucidate in vitro responses to combined gamma knife irradiation and p53 gene transfection on human malignant glioma cell lines. METHODS: Two malignant... -
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