Korean J Anat.  2003 Jun;36(3):159-166.

Apoptosis and Ultrastructural Changes of Glomerular Endothelial Cells of Mice with Streptozotocin-induced Diabetic Nephropathy

• Kim JS

Abstract

Diabetic nephropathy (DN) in mice induced by intraperitoneal injections of streptozotocin (STZ) three times at divided dosages; the first dosage of 50 mg/kg STZ was given to mice after 12-h fasting, followed by the second 50mg/kg STZ at 24-h after the first injection, and the third dosage of 100 mg/kg was given at 72-h after the first injection. The plasma glucose and creatinine levels gradually increased after the STZ treatment, indicating the onset of DB. Histopathological examination of the kidneys of mice treated with STZ revealed focal areas of mesangial proliferation with increased periodic acid-Schiff-positive materials. At 4 and 6 weeks diabetic groups after STZ treatment showed severely degenerated nuclei of the mesangial and endothelial cells. These results indicate that apoptic histopathological changes occurs in the kidney of the STZ-induced diabetic mellitus.

Keyword

Nephropathy; Glomerulus; Apoptosis; Ultrastructure

MeSH Terms

Animals
Apoptosis*
Blood Glucose
Creatinine
Diabetic Nephropathies*
Endothelial Cells*
Fasting
Injections, Intraperitoneal
Kidney
Mice*
Streptozocin
Creatinine
Streptozocin