J Korean Assoc Oral Maxillofac Surg.  2009 Aug;35(4):199-204.

Xenografted Tumorigenesis in the oral vestibule of nude mice by Snail transfection: Histological and immunohistochemical study

Affiliations
  • 1Department of Oral and Maxillofacial Surgery, National Health Insurance Corporation Ilsan Hospital, Korea. cha8764@yumc.yonsei.ac.kr
  • 2Oral Cancer Research Institute, Korea.
  • 3Department of Oral and Maxillofacial Surgery, Korea.
  • 4Department of Oral Pathology, College of Dentistry, Yonsei University, Korea.
  • 5Department of Dental Science, Graduate School, Yonsei University, Korea.

Abstract

PURPOSE
The purpose of this study is to investigate the epithelial-mesenchymal transition (EMT) induced by Snail transcription factor and Snailtransfected in vivo tumors with histopathological features.
MATERIALS AND METHODS
We induced in vivo xenografted tumorigenesis in the oral vestibules of nude mice by a Snail transfected HaCaT cell line and investigated morphological and immunohistochemical features in Snail expressive tumors.
RESULTS
We identified tumor masses in 14 out of 15 nude mice in the HaCaT-Snail cell inoculation group, but no tumors were present in any of the HaCaT cell inoculation group. Induced tumors showed features of poorly differentiated carcinoma with invasion to neighboring muscles and bones. The HaCaT-Snail tumors showed decreased expressions of E-cadherin and cytokeratin, but showed increased expressions of vimentin and N-cadherin. DISCUSSION: The Snail transfected xenograft can improve productivity of malignant tumors, show various histopathological features including invasive growth, and aid in the investigation of tumor progression and the interaction with surrounding tissues.

Keyword

E-cadherin; neoplastic cell transformation; Snail transcription factor; xenograft model
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