Toxicol Res.  2012 Dec;28(4):255-262.

The Chloroform Fraction of Carpinus tschonoskii Leaves Inhibits the Production of Inflammatory Mediators in HaCaT Keratinocytes and RAW264.7 Macrophages

• Kang GJ
• Kang NJ
• Han SC
• Koo DH
• Kang HK
• Yoo BS
• Yoo ES

Abstract

Inflammation is the immune system's response to infection and injury-related disorders, and is related to pro-inflammatory factors (NO, PGE2, cytokines, etc.) produced by inflammatory cells. Atopic dermatitis (AD) is a representative inflammatory skin disease that is characterized by increasing serum levels of inflammatory chemokines, including macrophage-derived chemokine (MDC). Carpinus tschonoskii is a member of the genus Carpinus. We investigated the anti-inflammatory activity of C. tschonoskii by studying the effects of various solvent fractions prepared from its leaves on inflammatory mediators in HaCaT and RAW264.7 cells. We found that the chloroform fraction of C. tschonoskii inhibited MDC at both the protein and mRNA levels in HaCaT cells, acting via the inhibition of STAT1 in the IFN-gamma signaling pathway. In addition, the chloroform fraction significantly suppressed the expression of inflammatory factors induced by lipopolysaccharide stimulation, except COX-2 and TNF-alpha. These results suggest that the chloroform fraction of C. tschonoskii leaves may include a component with potential anti-inflammatory activity.

Keyword

Carpinus tschonoskii; Inflammation mediators; HaCaT keratinocytes; RAW264.7 macrophages

MeSH Terms

Betulaceae
Chemokine CCL22
Chemokines
Chloroform
Cytokines
Dermatitis, Atopic
Dinoprostone
Inflammation
Inflammation Mediators
Keratinocytes
Macrophages
RNA, Messenger
Skin Diseases
Tumor Necrosis Factor-alpha
Chemokine CCL22
Chemokines
Chloroform
Cytokines
Dinoprostone
Inflammation Mediators
RNA, Messenger
Tumor Necrosis Factor-alpha