J Korean Med Sci.  2003 Feb;18(1):108-111. 10.3346/jkms.2003.18.1.108.

Distribution of Adenoviral Vector in Brain after Intravenous Administration

Affiliations
  • 1Department of Internal Medicine, Holy Family Hospital, College of Medicine, The Catholic University of Korea, Bucheon, Korea. jyjin@hfh.cuk.ac.kr
  • 2Division of Cardiology, Department of Internal Medicine, School of Medicine, Eulji University, Seoul, Korea.
  • 3Department of Neuroscience, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, U.S.A.
  • 4Department of Biology, Our Lady of Mercy Hospital, Korea.
  • 5Department of Pediatrics, Our Lady of Mercy Hospital, Korea.

Abstract

The delivery of transgenes to the central nervous system (CNS) can be a valuable tool to treat CNS diseases. Various systems for the delivery to the CNS have been developed; vascular delivery of viral vectors being most recent. Here, we investigated gene transfer to the CNS by intravenous injection of recombinant adenoviral vectors, containing green fluorescence protein (GFP) as a reporter gene. Expression of GFP was first observed 6 days after the gene transfer, peaked at 14 days, and almost diminished after 28 days. The observed expression of GFP in the CNS was highly localized to hippocampal CA regions of cerebral neocortex, inferior colliculus of midbrain, and granular cell and Purkinje cell layers of cerebellum. It is concluded that intravenous delivery of adenoviral vectors can be used for gene delivery to the CNS, and hence the technique could be beneficial to gene therapy.

Keyword

Adenoviridae; Central Nervous System; Green Fluorescent Protein; Gene Therapy

MeSH Terms

Adenoviruses, Human/isolation & purification*
Animals
Blood-Brain Barrier
Brain/virology*
Cerebellum/cytology
Cerebellum/virology
Comparative Study
Female
Genes, Reporter
Genetic Vectors/administration & dosage
Genetic Vectors/isolation & purification
Genetic Vectors/pharmacokinetics*
Hippocampus/virology
Inferior Colliculus/virology
Injections, Intravenous
Luminescent Proteins/analysis
Luminescent Proteins/biosynthesis
Luminescent Proteins/genetics
Mice
Mice, Inbred BALB C
Neuroglia/virology
Neurons/virology*
Purkinje Cells/virology
Pyramidal Cells/virology
Recombinant Fusion Proteins/analysis
Recombinant Fusion Proteins/biosynthesis
Recombinant Fusion Proteins/genetics
Tail/blood supply
Tissue Distribution
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