J Korean Soc Endocrinol.  2005 Aug;20(4):301-305. 10.3803/jkes.2005.20.4.301.

Endocrine Regeneration Therapy using Adenoviral vector

  • 1Endocrinology, Metabolism & Molecular Medicine Northwestern University, Feinberg School of Medicine 303 East Superior Street, USA.


No Abstract available.

MeSH Terms



  • Fig. 1 Colocalization of prolactin (PRL) with stem cell markers in livers of mice treated with Ad-Pit-1. Paraffin sections of livers of mice at day 5 after treatment were analyzed by double immunofluorescence staining for PRL with c-kit, thy-1, or cytokeratin 14. Cells with red fluorescent cytoplasm express PRL (yellow arrow in left panel) and cells with green fluorescent cytoplasm (red arrow in center panel) express c-kit (upper), thy-1 (middle) or cytokeratin 14 (lower). PV: Portal vein. Upper and middle panels are 630 × magnification and lower panel is 1000 × magnification. Lactotrope cells express hepatic stem cells marker at the initial stage of differentiation. Reprinted from (8) with permission.

  • Fig. 2 Adenoviral vector and Ad/AAV hybrid vectors carrying gene of interest. Infection of cells with Ad/AAV hybrid vector enables precise excision of the AAV inverted terminal repeat (ITR)-flanked gene from adenoviral genome, subsequently integration into the host genome occurs. In the presence of Rep78 expression unit, transgene is predictably integrated into the AAVS1 locus on human chromosome 19.

  • Fig. 3 GFP expression in HepG2 cells infected with adenovirus or Ad/AAV hybrid vectors carrying pancreas-duodenum homeobox 1 (PDX1)-internal ribosomal entry site (IRES)-green fluorescent protein (GFP). GFP was more abundantly expressed in HepG2 cells infected with Ad/AAV hybrid vector. (not published data)


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