Restor Dent Endod.  2020 Feb;45(1):e2. 10.5395/rde.2020.45.e2.

Cytocompatibility and cell proliferation evaluation of calcium phosphate-based root canal sealers

• Mestieri L
• Zaccara IM
• Pinheiro L
• Barletta F
• Kopper PM
• Grecca FS

• KMID: 2470338
• DOI: http://doi.org/10.5395/rde.2020.45.e2

Abstract

OBJECTIVES
This study aimed to evaluate the cell viability and migration of Endosequence Bioceramic Root Canal Sealer (BC Sealer) compared to MTA Fillapex and AH Plus.
MATERIALS AND METHODS
BC Sealer, MTA Fillapex, and AH Plus were placed in contact with culture medium to obtain sealers extracts in dilution 1:1, 1:2 and 1:4. 3T3 cells were plated and exposed to the extracts. Cell viability and migration were assessed by 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide (MTT) and Scratch assay, respectively. Data were analyzed by Kruskal-Wallis and Dunn's test (p < 0.05).
RESULTS
The MTT assay revealed greater cytotoxicity for AH Plus and MTA Fillapex at 1:1 dilution when compared to control (p < 0.05). At 1:2 and 1:4 dilutions, all sealers were similar to control (p > 0.05) and MTA Fillapex was more cytotoxic than BC Sealer (p < 0.05). Scratch assay demonstrated the continuous closure of the wound according to time. At 30 hours, the control group presented closure of the wound (p < 0.05). At 36 hours, only BC Sealer presented the closure when compared to AH Plus and MTA Fillapex (p < 0.05). At 42 hours, AH Plus and MTA Fillapex showed a wound healing (p > 0.05).
CONCLUSIONS
All tested sealers demonstrated cell viability highlighting BC Sealer, which showed increased cell migration capacity suggesting that this sealer may achieve better tissue repair when compared to other tested sealers.

Keyword

Root canal; Cell migration; Fibroblast; Endodontics

MeSH Terms

3T3 Cells
Animals
Calcium*
Cell Movement
Cell Proliferation*
Cell Survival
Dental Pulp Cavity*
Endodontics
Fibroblasts
Mice
Pemetrexed
Wound Healing
Wounds and Injuries
Calcium
Pemetrexed

Figure

• 

  Figure 1. Cell viability results (%) by 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide (MTT) assay in 3T3 cells exposed to 24 hours for AH Plus, MTA Fillapex and BC Sealer, at dilution of 1:1, 1:2 and 1:4, according to the control group (100% viability rate). Bars with different letters represent significant differences among groups in each dilution extract (p < 0.05).

• 

  Figure 2. Cell migration results by scratch assay in 3T3 cells exposed to set extracts of AH Plus, MTA Fillapex and BC Sealer at dilution of 1:8. Percentage of open area by scratch assay in 3T3 cells. Columns with different letters represent significant differences between groups in each evaluated period.

• 

  Figure 3. Illustrative image of scratch assay in 3T3 cells.

Reference

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