J Pathol Transl Med.  2019 Jul;53(4):217-224. 10.4132/jptm.2019.02.20.

Association between Expression of 8-OHdG and Cigarette Smoking in Non-small Cell Lung Cancer

• An AR
• Kim KM
• Park HS
• Jang KY
• Moon WS
• Kang MJ
• Lee YC
• Kim JH
• Chae HJ
• Chung MJ

• KMID: 2454601
• DOI: http://doi.org/10.4132/jptm.2019.02.20

Abstract

BACKGROUND
Exposure to cigarette smoking (CS) is a major risk factor for the development of lung cancer. CS is known to cause oxidative DNA damage and mutation of tumor-related genes, and these factors are involved in carcinogenesis. 8-Hydroxydeoxyguanosine (8-OHdG) is considered to be a reliable biomarker for oxidative DNA damage. Increased levels of 8-OHdG are associated with a number of pathological conditions, including cancer. There are no reports on the expression of 8-OHdG by immunohistochemistry in non-small cell lung cancer (NSCLC).
METHODS
We investigated the expression of 8-OHdG and p53 in 203 NSCLC tissues using immunohistochemistry and correlated it with clinicopathological features including smoking.
RESULTS
The expression of 8-OHdG was observed in 83.3% of NSCLC. It was significantly correlated with a low T category, negative lymph node status, never-smoker, and longer overall survival (p < .05) by univariate analysis. But multivariate analysis revealed that 8-OHdG was not an independent prognostic factor for overall survival in NSCLC patients. The aberrant expression of p53 significantly correlated with smoking, male, squamous cell carcinoma, and Ki-67 positivity (p < .05).
CONCLUSIONS
The expression of 8-OHdG was associated with good prognostic factors. It was positively correlated with never-smokers in NSCLC, suggesting that oxidative damage of DNA cannot be explained by smoking alone and may depend on complex control mechanisms.

Keyword

Carcinoma, non-small cell lung; 8-Oxo-7-hydrodeoxyguanosine; p53; Smoking; Immunohistochemistry

MeSH Terms

Carcinogenesis
Carcinoma, Non-Small-Cell Lung*
Carcinoma, Squamous Cell
DNA
DNA Damage
Humans
Immunohistochemistry
Lung Neoplasms
Lymph Nodes
Male
Multivariate Analysis
Risk Factors
Smoke
Smoking*
Tobacco Products*
DNA
Smoke

Figure

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  Fig. 1. Immunohistochemical expression of 8-hydroxydeoxyguanosine (8-OHdG) in non-small cell carcinomas. Expression of 8-OHdG in nuclei of tumor cells. (A, B) Adenocarcinoma. (C, D) Squamous cell carcinoma.

• 

  Fig. 2. Kaplan-Meier survival analysis of non-small cell lung carcinoma patients. Overall survival in 203 patients according to the expression of 8-hydroxydeoxyguanosine (8-OHdG) (A), p53 (B), Ki-67 (C), smoking history (D), and T category (American Joint Committee on Cancer (AJCC) 8th edition) (E).

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