Biomol Ther.  2019 Feb;27(2):210-215. 10.4062/biomolther.2018.054.

Gomisin G Suppresses the Growth of Colon Cancer Cells by Attenuation of AKT Phosphorylation and Arrest of Cell Cycle Progression

Affiliations
  • 1Center for Medical Science Research, College of Medicine, Hallym University, Chuncheon 24252, Republic of Korea. hjookwon@hallym.ac.kr
  • 2Division of Bioscience and Biotechnology, BMIC, Konkuk University, Seoul 05029, Republic of Korea.
  • 3Department of Biomedical Science, College of Natural Science, Hallym University, Chuncheon 24252, Republic of Korea.
  • 4Department of Biochemistry, College of Natural Sciences, Chungbuk National University, Cheongju 28644, Republic of Korea.
  • 5Department of Microbiology, College of Medicine, Hallym University, Chuncheon 24252, Republic of Korea.

Abstract

Colorectal cancer is one of the leading causes of cancer related death due to a poor prognosis. In this study, we investigated the effect of Gomisin G on colon cancer growth and examined the underlying mechanism of action. We found that Gomisin G significantly suppressed the viability and colony formation of LoVo cells. Gomisin G reduced the phosphorylation level of AKT implying that Gomisin G suppressed the PI3K-AKT signaling pathway. Gomisin G also induced apoptosis shown by Annexin V staining and an increased level of cleaved poly-ADP ribose polymerase (PARP) and Caspase-3 proteins. Furthermore, Gomisin G remarkably triggered the accumulation of cells at the sub-G1 phase which represents apoptotic cells. In addition, the level of cyclin D1 and phosphorylated retinoblastoma tumor suppressor protein (Rb) was also reduced by the treatment with Gomisin G thus curtailing cell cycle progression. These findings show the suppressive effect of Gomisin G by inhibiting proliferation and inducing apoptosis in LoVo cells. Taken together, these results suggest Gomisin G could be developed as a potential therapeutic compound against colon cancer.

Keyword

Gomisin G; Colon cancer; AKT; Apoptosis; PARP; Cell cycle

MeSH Terms

Annexin A5
Apoptosis
Caspase 3
Cell Cycle*
Colon*
Colonic Neoplasms*
Colorectal Neoplasms
Cyclin D1
Phosphorylation*
Prognosis
Retinoblastoma
Ribose
Annexin A5
Caspase 3
Cyclin D1
Ribose
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