J Korean Soc Clin Toxicol.  2018 Jun;16(1):9-14. 10.22537/jksct.2018.16.1.9.

The Effect of Intravenous Lipid Emulsion in a Rat Model of Verapamil Toxicity

• Ha DM
• Kim DH
• Kim T
• Lee SH
• Jeong JH
• Lee SB
• Lim D
• Kang C

• KMID: 2436259
• DOI: http://doi.org/10.22537/jksct.2018.16.1.9

Abstract

PURPOSE
Intravenous lipid emulsion (ILE) has been shown to have significant therapeutic effects on calcium channel blocker overdose in animal studies and clinical cases. In this preliminary experiment, we investigated the hemodynamic changes and survival in a rat model of verapamil intoxication.
METHODS
Fourteen male Sprague-Dawley rats were sedated and treated with ILE or normal saline (control), followed by continuous intravenous infusion of verapamil (20 mg/kg/h). Mean arterial pressure and heart rate of rats were monitored during the infusion. In addition, the total dose of infused verapamil and the duration of survival were measured.
RESULTS
Survival was prolonged in the ILE group (32.43±5.8 min) relative to the control group (24.14±4.3 min) (p=0.01). The cumulative mean lethal dose of verapamil was higher in the ILE group (4.3±0.7 mg/kg) than in the control group (3.2±0.5 mg/kg; p=0.017).
CONCLUSION
ILE pretreatment prolonged survival and increased the lethal dose in a rat model of verapamil poisoning.

Keyword

Intravenous lipid emulsion (ILE); Verapamil; Intoxication; Poisoning

MeSH Terms

Animals
Arterial Pressure
Calcium Channels
Heart Rate
Hemodynamics
Humans
Infusions, Intravenous
Male
Models, Animal*
Poisoning
Rats*
Rats, Sprague-Dawley
Therapeutic Uses
Verapamil*
Calcium Channels
Therapeutic Uses
Verapamil