World J Mens Health.  2018 May;36(2):153-160. 10.5534/wjmh.17010.

Improvement of Persistent Detrusor Overactivity through Treatment with a Phytotherapeutic Agent (WSY-1075) after Relief of Bladder Outlet Obstruction

Affiliations
  • 1Department of Urology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea. ksw1227@catholic.ac.kr
  • 2Department of Urology, Institute of Biomedical Industry, The Catholic University of Korea, Seoul, Korea.
  • 3Catholic Integrative Medicine Research Institute, College of Medicine, The Catholic University of Korea, Seoul, Korea.
  • 4Korea Biomedical Science Institute, Seoul, Korea.

Abstract

PURPOSE
Many patients with benign prostatic hyperplasia need treatment for remaining storage symptoms after surgery. Therefore, we evaluated the effect of the phytotherapeutic agent WSY-1075 on persistent detrusor overactivity (DO) after the relief of bladder outlet obstruction (BOO).
MATERIALS AND METHODS
Rats were assigned to 3 groups: control (n=6), persistent DO (n=6), and persistent DO treated with the phytotherapeutic agent WSY-1075 (n=6). Persistent DO after relief of partial BOO was generated in the rat model, and 6 of the rats with this condition were orally administered WSY-1075. After 4 weeks of administration, cystometry was performed. Additionally, 8-hydroxy-2-deoxyguanosine and superoxide dismutase were measured to evaluate oxidative stress in the bladder. Pro-inflammatory cytokines, such as interleukin-8 and tumor necrosis factor-α, were analyzed, as were the M2 and M3 muscarinic receptors of the bladder.
RESULTS
Significantly increased contraction pressure and a decreased contraction interval were observed in the persistent DO group after relief of BOO. Moreover, oxidative stress, pro-inflammatory cytokines, and M3 muscarinic receptors were significantly increased. After treatment with WSY-1075, significantly reduced DO was observed by cystometry in comparison with the persistent DO group. Additionally, significantly decreased levels of oxidative stress, pro-inflammatory cytokines, and M3 muscarinic receptors in the bladder were observed after treatment with WSY-1075.
CONCLUSIONS
Treatment with WSY-1075 improved persistent DO after the relief of BOO mediated by antioxidative and anti-inflammatory effects. Further studies are necessary to identify the exact mechanism of the treatment effect of WSY-1075.

Keyword

Lower urinary tract symptoms; Phytotherapy; Prostatic hyperplasia; Urinary bladder, overactive

MeSH Terms

Animals
Cytokines
Humans
Interleukin-8
Lower Urinary Tract Symptoms
Models, Animal
Necrosis
Oxidative Stress
Phytotherapy
Prostatic Hyperplasia
Rats
Receptors, Muscarinic
Superoxide Dismutase
Urinary Bladder Neck Obstruction*
Urinary Bladder*
Urinary Bladder, Overactive
Cytokines
Interleukin-8
Receptors, Muscarinic
Superoxide Dismutase

Figure

  • Fig. 1 Representative cystometric curves. (A) Control group. (B) Persistent detrusor overactivity (DO) group. (C) Persistent DO with treatment group.

  • Fig. 2 Changes in 8-OHdG and SOD. Values are expressed as means±standard deviations. 8OHdG: 8-hydroxy-2-deoxyguanosine, SOD: superoxide dismutase, DO: detrusor overactivity. ap<0.05 compared with the control group; bp<0.05 compared with the persistent DO group.

  • Fig. 3 Changes in pro-inflammatory cytokines. Values are expressed as means±standard deviations. IL-8: interleukin-8, TNF-α: tumor necrosis factor-α, DO: detrusor overactivity. ap<0.05 compared with the control group; bp<0.05 compared with the persistent DO group.

  • Fig. 4 Changes in the M2 and M3 muscarinic receptors. Values are expressed as means±standard deviations. DO: detrusor overactivity. ap<0.05 compared with the control group; bp<0.05 compared with the persistent DO group.

  • Fig. 5 Improvement of persistent detrusor overactivity (DO) after relief of bladder outlet obstruction (BOO) mediated by the antioxidative and anti-inflammatory effects of WSY-1075.


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