Immune Netw.  2018 Apr;18(2):e9. 10.4110/in.2018.18.e9.

Optimization of Cytokine Milieu to Reproduce Atopic Dermatitis-related Gene Expression in HaCaT Keratinocyte Cell Line

Affiliations
  • 1Department of Dermatology, Gachon Gil Medical Center, School of Medicine, Gachon University, Incheon 21565, Korea. jyroh1@gilhospital.com
  • 2Department of Microbiology, School of Medicine, Gachon University, Incheon 21565, Korea. yjjung@gachon.ac.kr
  • 3Gachon Advanced Institute for Health Science & Technology, School of Medicine, Gachon University, Incheon 21565, Korea.

Abstract

Although atopic dermatitis (AD) is characterized by cytokine production predominantly mediated by T helper (Th) 2 cells, AD pathogenesis also involves innate immune and Th1 cells. To optimize the cytokine milieu required for accurate reproduction of AD-related gene expression profile in vitro, we evaluated the expression pattern of CCL22, CCL17, IL5, IL13, IL33, IL25, TSLP, FLG, and LOR in human lesional AD skin and cytokine-stimulated HaCaT cells. An increase in Th2 mediators (IL5, IL13, CCL22, CCL17, IL25, IL33, and TSLP) and a decrease in genes related to cornified cell envelope (filaggrin and loricrin) were observed in human AD lesions. Innate (tumor necrosis factor-α) and/or Th1/Th2 adaptive cytokines (interferon-γ/IL-4) were required for inducing these inflammatory changes in HaCaT cells, implying that a complex network of innate, Th1, and Th2 cytokines drives AD-like changes. Therefore, stimulation with various combinations of cytokines, beyond Th2 polarization, is necessary when HaCaT cell line is used to study genetic changes implicated in AD pathogenesis.

Keyword

Atopic dermatitis; Cytokine; In vitro stimulation

MeSH Terms

Cell Line*
Cytokines
Dermatitis, Atopic
Gene Expression*
Humans
In Vitro Techniques
Interleukin-13
Interleukin-33
Interleukin-5
Keratinocytes*
Necrosis
Reproduction
Skin
Th1 Cells
Transcriptome
Cytokines
Interleukin-13
Interleukin-33
Interleukin-5
Full Text Links
  • IN
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
    DB Error: unknown error