Lab Anim Res.  2017 Jun;33(2):179-186. 10.5625/lar.2017.33.2.179.

Comparative study of fertilization rates of C57BL/6NKorl and C57BL/6N mice obtained from two other sources

Affiliations
  • 1College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, Korea. dyhwang@pusan.ac.kr
  • 2Department of Pharmacy, College of Pharmacy, Pusan National University, Busan, Korea.
  • 3Departmentof Microbiology and Immunology, Inje University College of Medicine, Busan, Korea.
  • 4Department of Health and Exercise Science, Korea National Sport University, Seoul, Korea.
  • 5College of Veterinary Medicine, Kyungpook National University, Daegu, Korea.

Abstract

C57BL/6N is the most widely used inbred mouse strain applied in a wide variety of research areas including cancer, cardiovascular biology, developmental biology, diabetes and obesity, genetics, immunology, neurobiology, and sensorineural research. To compare the fertilization rates of C57BL/6NKorl mice with two commercial C57BL/6N stocks, differences in reproductive organ structures, sperm and egg numbers, fertilization rates, and embryo development rates among C57BL/6NKorl (Korea FDA source), C57BL/6NA (USA source), and C57BL/6NB (Japan source) mice were determined. Among the stocks, no significant differences were detected in organ weight and histological structure of male and female reproductive organs, although body weight was higher in C57BL/6NKorl mice than that in the other groups. The concentration and morphology of sperm and eggs in C57BL/6NKorl mice were similar to those of C57BL/6NA and C57BL/6NB mice. Furthermore, the three stocks had similar in vitro fertilization and embryo development rates, although these rates tended to be higher in C57BL/6NB mice. Pup body weight was higher in C57BL/6NKorl and C57BL/6NB mice than that in C57BL/6NA mice. The results of the present study suggest that C57BL/6NKorl, C57BL/6NA, and C57BL/6NB mice obtained from three different sources have similar fertilization and embryo development rates, although there were slight differences in the magnitude of their responses rates.

Keyword

C57BL/6NKorl; reproductive ability; sperm; egg; in vitro fertilization; embryo development rate

MeSH Terms

Allergy and Immunology
Animals
Biology
Body Weight
Developmental Biology
Eggs
Embryonic Development
Female
Fertilization in Vitro
Fertilization*
Genetics
Humans
Male
Mice*
Mice, Inbred Strains
Neurobiology
Obesity
Organ Size
Ovum
Pregnancy
Spermatozoa
Full Text Links
  • LAR
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
    DB Error: unknown error