Tissue Eng Regen Med.  2018 Feb;15(1):89-100. 10.1007/s13770-017-0088-4.

Implantation of Bone Marrow Stromal Cell Sheets Derived from Old Donors Supports Bone Tissue Formation

Affiliations
  • 1Department of Public Health, Health Management and Policy, Faculty of Medicine, Nara Medical University, Shijo 840, Kashihara, Nara 634-8521, Japan. makahane@naramed-u.ac.jp
  • 2Department of Orthopedic Surgery, Nara Medical University, Shijo 840, Kashihara, Nara 634-8522, Japan.
  • 3Department of Arthroplasty and Regenerative Medicine, Nara Medical University, Shijo 840, Kashihara, Nara 634-8522, Japan.

Abstract

The purpose of this study was to evaluate the osteogenesis ability of osteogenic matrix cell sheets (OMCS) derived from old donor cells. Bone marrow stromal cells (BMSC) were obtained from young (7-week-old) and old (1-year-old) Fischer344 rats donors and cultured with modified Eagle's medium (MEM group) alone or containing dexamethasone (Dex; 10 nM) and ascorbic acid phosphate (AscP; 0.28 mM) (Dex/AscP group). We prepared four in vitro experimental groups: (1) young MEM, (2) young Dex/AscP, (3) old MEM and (4) old Dex/AscP. Cell proliferation and osteogenic marker mRNA expression levels were evaluated in vitro. To assess bone formation in vivo, the cells of each group were combined with beta tricalcium phosphate (TCP) disks followed by implantation in recipient rats. The in vitro study showed significant differences in the mRNA expression of osteocalcin, ALP, and BMP2 between MEM and Dex/AscP groups. Bone formation following implantation was observed upon histological analyses of all groups. TCP combined with OMCS (OMCS/TCP group) resulted in enhanced bone formation compared to that following combination with BMSC (BMSC/TCP). The osteocalcin content of the OMCS/TCP group 4 weeks after implantation was significantly higher than that in the BMSC/TCP construct for both young and old donors. The present study clearly indicated that OMCS could be generated from BMSCs of old as well as young donors using a mechanical retrieval method. Thus, through its usage of OMCS, this method may represent a potentially effective therapeutic option for cell-based therapy in elderly patients.

Keyword

Old donor; Cell sheet; Osteogenesis; Beta tricalcium phosphate

MeSH Terms

Aged
Animals
Ascorbic Acid
Bone and Bones*
Bone Marrow*
Cell Proliferation
Dexamethasone
Humans
In Vitro Techniques
Mesenchymal Stromal Cells*
Methods
Osteocalcin
Osteogenesis
Rats
RNA, Messenger
Tissue Donors*
Ascorbic Acid
Dexamethasone
Osteocalcin
RNA, Messenger
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