J Korean Assoc Maxillofac Plast Reconstr Surg.  2010 Mar;32(2):97-106.

BONE REGENERATION WITH ADIPOSE TISSUE-DERIVED MESENCHYMAL STEM CELL AND HA/TCP

Affiliations
  • 1Department of Oral and Maxillofacial Surgery, College of Medicine, Korea University, Korea. jaesrim@korea.ac.kr

Abstract

AIM OF THE STUDY: An alternative source of adult stem cells that could be obtained in large quantities, under local anesthesia, with minimal discomfort would be advantageous. Adipose tissue could be processed to obtain a fibroblast-like population of cells or adipose tissue-derived stromal cells (ATSCs). This study was performed to confirm the availability of ATSCs in bone tissue engineering. MATERIALS & METHODS: In this study, adipose tissue-derived mesenchymal stem cell was extracted from the liposuctioned abdominal fat of 24-old human and cultivated, and the stem cell surface markers of CD 105 and SCF-R were confirmed by immunofluorescent staining. The proliferation of bone marrow mesenchymal stem cell and ATSCs were compared, and evaluated the osteogenic differentiation of ATSCs in a specific osteogenic induction medium. Osteogenic differentiation was assessed by von Kossa and alkaline phosphatase staining. Expression of osteocyte specific BMP-2, ALP, Cbfa-1, Osteopontin and osteocalcin were confirmed by RT-PCR. With differentiation of ATSCs, calcium concentration was assayed, and osteocalcin was evaluated by ELISA (Enzyme-linked immunosorbant assay). The bone formation by 5-week implantation of HA/TCP block loaded with bone marrow mesenchymal stem cells and ATSCs in the subcutaneous pocket of nude mouse was evaluated by histologic analysis.
RESULTS
ATSCs incubated in the osteogenic medium were stained positively for von Kossa and alkaline phosphatase staining. Expression of osteocyte specific genes was also detected. ATSCs could be easily identified through fluorescence microscopy, and bone formation in vivo was confirmed by using ATSC-loaded HA/TCP scaffold.
CONCLUSIONS
The present results show that ATSCs have an ability to differentiate into osteoblasts and formed bone in vitro and in vivo. So ATSCs may be an ideal source for further experiments on stem cell biology and bone tissue engineering.

Keyword

Adipose tissue-derived stromal cells (ATSCs); Bone tissue engineering; Osteogenic medium

MeSH Terms

Abdominal Fat
Adipose Tissue
Adult Stem Cells
Alkaline Phosphatase
Anesthesia, Local
Animals
Biology
Bone and Bones
Bone Marrow
Bone Regeneration
Calcium
Durapatite
Enzyme-Linked Immunosorbent Assay
Humans
Mesenchymal Stromal Cells
Mice
Mice, Nude
Microscopy, Fluorescence
Osteoblasts
Osteocalcin
Osteocytes
Osteogenesis
Osteopontin
Stem Cells
Stromal Cells
Alkaline Phosphatase
Calcium
Durapatite
Osteocalcin
Osteopontin
Full Text Links
  • JKAMPRS
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr