J Korean Orthop Assoc.  2003 Oct;38(6):624-630.

Effect of Polyethylene Particles on Osteoblasts in Pathogenesis of Osteolysis

• Lee WS
• Han CD
• Kim SJ
• Kang ES
• Park JC

Abstract

PURPOSE
To determine the role of osteoblast in the pathogenesis of osteolysis, we evaluated cell proliferation and differentiation of culturedosteoblasts by adding retrieved polyethylene particles. MATERIALS AND METHODS: We cultured the osteoblasts primarily isolated from rat neonate calvariums and then added polyethylene particles retrieved from osteolysis tissue. We evaluated cell proliferation, alkaline phosphatase activity, the expression of Type I procollagen mRNA, the expression of osteocalcin mRNA, bone nodule formation, and the expression of osteoprotegerin (OPG) mRNA and receptor activatorof NF-kappa B ligand (RANKL) mRNA according to culture periods. RESULTS: Particle groups showed statistically significant decrease in cell proliferation after the 7th day. The expression of type I procollagen mRNA increased on the 14th day. The bone nodules appeared at the 7th day and increased with time. There was no appearance of bone nodule in the control group. The expression of OPG mRNA in particle groups was not changed. The expression of RANKL mRNA was significantly increased in high concentration particle group (4 mg/mL) on the 14th day. CONCLUSION: Polyethylene particles ceased the proliferation of osteoblasts and produced early extracellular matrix maturation and mineralization process in vitro and increased the expression of RANKL promoting osteoclast activities.

Keyword

Polyethylene particles; Osteoblast; Growth; Differentiation; Osteoprotegerin; Receptoractivator of NF-kappa B ligand

MeSH Terms

Alkaline Phosphatase
Animals
Cell Proliferation
Collagen Type I
Extracellular Matrix
Humans
Infant, Newborn
NF-kappa B
Osteoblasts*
Osteocalcin
Osteoclasts
Osteolysis*
Osteoprotegerin
Polyethylene*
Rats
RNA, Messenger
Skull
Alkaline Phosphatase
Collagen Type I
NF-kappa B
Osteocalcin
Osteoprotegerin
Polyethylene
RNA, Messenger