Tissue Eng Regen Med.  2016 Aug;13(4):388-395. 10.1007/s13770-015-0104-5.

Inhibition of Fibrotic Contraction by C-Phycocyanin through Modulation of Connective Tissue Growth Factor and α-Smooth Muscle Actin Expression

Affiliations
  • 1College of Pharmacy, Duksung Women's University, Seoul, Korea. aeri@ds.ac.kr

Abstract

The effects of C-phycocyanin (C-pc), a phycobiliprotein, on the expression of pro-fibrotic mediators in hyper-tropic scarring such as connective tissue growth factor (CTGF) and α-smooth muscle actins (α-SMA) were investigated in relation to trans-differentiation of fibroblast to myo-fibroblast, an icon of scar formation. C-pc was isolated from Spirulina Platensis extract using sonication method and C-pc concentration was determined by Bennet and Bogorad equation. α-SMA and CTGF levels in wounded primary human dermal fibroblasts were determined by western blot analysis and immuno-fluorescence confocal microscope was employed. Fibroblast contractility was examined by three-dimensional collagen lattice contraction assay. There was an elevation of α-SMA (121%) and CTGF (143%) levels in wound cells as compared with non-wound cells. The does-response profiles of down regulation demonstrated that the maximum inhibitions of α-SMA by 63% (p<0.05) and CTGF by 50% (p<0.1) were achieved by C-pc (6 nM) treated cells. In confocal assay, non-wound fibroblasts exhibited basal level of α-SMA staining, while wounded cells without C-pc treatment showed strong up-regulation of α-SMA by 147% (p<0.05). C-pc (6 nM) inhibited α-SMA expression by 70% (p<0.05) and reduced collagen contraction by 29% (p<0.05). C-pc seemed to lessen the over expression of CTGF, α-SMA, subsequently alleviating the fibrotic contracture. This study suggests the potential application of C-pc to regulation of the expression of pro-fibrotic mediators in scarring process and its potential usage as an efficient means for anti-fibrosis therapy.

Keyword

Phycocyanin; Wound fibrosis; Myofibroblast; α-SMA; Connective tissue growth factor; Wound healing

MeSH Terms

Actins*
Blotting, Western
Cicatrix
Collagen
Connective Tissue Growth Factor*
Connective Tissue*
Contracture
Down-Regulation
Fibroblasts
Humans
Methods
Myofibroblasts
Phycocyanin*
Sonication
Spirulina
Up-Regulation
Wound Healing
Wounds and Injuries
Actins
Collagen
Connective Tissue Growth Factor
Phycocyanin
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