J Korean Ophthalmol Soc.
2017 Mar;58(3):327-332. 10.3341/jkos.2017.58.3.327.
Effect of Trypan Blue on the Survival of Cultured Trabecular Meshwork Cells
- Affiliations
-
- 1Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr
- KMID: 2371914
- DOI: http://doi.org/10.3341/jkos.2017.58.3.327
Abstract
- PURPOSE
To evaluate the effects of trypan blue (TB) on the survival of cultured human trabecular meshwork cells (HTMCs).
METHODS
Primarily cultured HTMCs were exposed to 0.05, 0.10 or 0.50% TB for 1, 5 or 30 min. Cellular survival was assessed using the MTT assay and degree of apoptosis was analyzed with flow cytometry using annexin-V/propidium iodide double staining.
RESULTS
Long-term exposure or high concentration of TB decreased the survival of HTMCs (p < 0.05). In flow cytometric analysis, exposure to 0.50% TB for 30 min increased the degree of apoptosis (p < 0.05). Commercial TB decreased cell survival after exposure for 5 min and increased the degree of apoptosis after exposure for 30 min (p < 0.05).
CONCLUSIONS
TB may cause cellular damage of cultured HTMCs and apoptosis could be the underlying mechanism. In TB-assisted cataract surgery, TB should be used for the shortest time possible and removed completely.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Effect of beta-adrenergics on the Survival and Production of Nitric Oxide in the Cultured Trabecular Meshwork Cells
- Effect of Mitomycin C on the Proliferation and Nitric Oxide Production in the Cultured Trabecular Meshwork Cells
- Distribution of proteoglycans in the human trabecular meshwork: histochemical electron microscopic findings with cuprolinic blue
- Effect of High Glucose on the Production of Reactive Oxygen Species in Trabecular Meshwork Cells
- Effect of Erythropoietin on the Production of Nitric Oxide in Trabecular Meshwork Cells
