J Korean Ophthalmol Soc.
2011 Dec;52(12):1514-1518. 10.3341/jkos.2011.52.12.1514.
Effect of Erythropoietin on the Production of Nitric Oxide in Trabecular Meshwork Cells
- Affiliations
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- 1Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea. jwkim@cu.ac.kr
- KMID: 2215158
- DOI: http://doi.org/10.3341/jkos.2011.52.12.1514
Abstract
- PURPOSE
To investigate the effects of erythropoietin (EPO) on the production of nitric oxide in cultured human trabecular meshwork cells (HTMC).
METHODS
Primarily cultured HTMC were exposed to 0, 0.5, and 1.0 U/ml EPO using serum-deprived media for 2 days. Production of nitric oxide and cellular survival were assessed with Griess assay and MTT assay, respectively. Expression of EPO mRNA receptor and activity of eNOS were assessed with RT-PCR.
RESULTS
EPO did not affect the survival of HTMC (p > 0.05). EPO increased the production of nitric oxide (p < 0.05) accompanied with increased eNOS activity.
CONCLUSIONS
EPO had no effect on the cellular survival under serum-deprived conditions. EPO increases the production of nitric oxide by increasing eNOS activity.
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