J Korean Ophthalmol Soc.  2010 Mar;51(3):418-422.

Effect of High Glucose on the Production of Reactive Oxygen Species in Trabecular Meshwork Cells

• Bae CB
• Kim JW

Abstract

PURPOSE
To investigate the effect of high glucose concentration on the production of reactive oxygen species in cultured human trabecular meshwork cells (HTMCs).
METHODS
Primarily cultured HTMCs were exposed to low glucose (5 mM) and high glucose (22 mM) concentrations, respectively, for seven days. Cellular survival, as well as nitric oxide (NO) and hydrogen peroxide production, were assessed by measured MTT assay, Griess assay, and o-Dianisidine dihydrochloride assay, respectively. Some cells were co-exposed to N-acetyl cysteine (NAC) to assess the effect of this antioxidant.
RESULTS
High glucose concentration increased the survival of cultured HTMCs significantly, with no effect from NAC. High glucose concentration increased the production of NO and hydrogen peroxide, which were abolished by co-exposure with NAC.
CONCLUSIONS
High glucose concentration increases the production of NO and hydrogen peroxide, which can be abolished by antioxidant in trabecular meshwork cells.

Keyword

High glucose; Hydrogen peroxide; Nitric oxide; Reactive oxygen species; Trabecular meshwork cells

MeSH Terms

Cysteine
Dianisidine
Glucose
Humans
Hydrogen Peroxide
Nitric Oxide
Reactive Oxygen Species
Trabecular Meshwork
Cysteine
Dianisidine
Glucose
Hydrogen Peroxide
Nitric Oxide
Reactive Oxygen Species

Figure

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  Figure 1. Effect of high glucose (HG) on the proliferation of human trabecular meshwork cells. HG increased cellular proliferation significantly compared to low glucose (LG) (* p<0.05). Co-exposed 50 or 100 μM N-acetylcysteine (NAC) did not affect the HG-induced increased cellular proliferation.

• 

  Figure 2. Effect of high glucose (HG) on the production of nitric oxide (NO) in primarily cultured human trabecular meshwork cells. HG increased NO production significantly compared to low glucose (LG) (* p<0.05), which was abolished by co-exposed 50 or 100 μM N-acetylcysteine (NAC) (**p<0.05).

• 

  Figure 3. Effect of high glucose (HG) on the production of hydrogen peroxide of human trabecular meshwork cells. HG increased hydrogen peroxide production significantly compared to low glucose (LG) (* p<0.05), which was abolished by co-exposed 50 or 100 μM N-acetylcysteine (NAC) (** p<0.05).

Reference

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