J Korean Med Sci.  2016 Jan;31(1):98-105. 10.3346/jkms.2016.31.1.98.

Biochemical Markers of Oxidative Stress in Saudi Women with Recurrent Miscarriage

• Ghneim HK
• Alshebly M

• KMID: 2359998
• DOI: http://doi.org/10.3346/jkms.2016.31.1.98

Abstract

This study was undertaken to investigate the antioxidant/oxidant status in recurrent miscarriage patients. Antioxidants including glutathione peroxidase (GPx), catalase (CAT), glutathione reductase (GR), reduced glutathione (GSH) and selenium (Se), as well as the oxidants hydrogen peroxide (H2O2), oxidised glutathione (GSSG) and lipid peroxidation were assayed in plasma, whole blood and placental tissue of non-pregnant women (NP), healthy pregnant women (HP), and recurrent miscarriage (RM) patients. Results indicated that all antioxidant activities and levels in plasma and whole blood of HP women were consistently moderately lower, and much more significantly lower in RM patients when both were compared to those seen in NP women (P<0.05 and P<0.001, respectively). Furthermore, whereas plasma antioxidant activities and levels were significantly lower in RM patients, those of whole blood and placental tissue were much more significantly lower when compared with HP women (P<0.001). Concurrent with these findings there were consistent increases of equal statistical significance and magnitude in the levels of all investigated oxidants assayed in all samples when compared in between subjects of the study as indicated above. Data thus illustrated a distinct shift in favor of oxidative reactions and reactive oxygen species (ROS) generation, and very significant decreases in the GSH/GSSG ratios in whole blood and placental tissue of RM patients when compared to HP and NP women (P<0.001). The above noted oxidative stress could have been a major causative factor of recurrent miscarriage.

Keyword

Abortion; Habitual; Oxidative Stress; Antioxidants; Oxidants; Placental Tissue; Blood

MeSH Terms

Abortion, Habitual/*blood/*epidemiology
Adult
Antioxidants/analysis
Biomarkers/*blood
Catalase/blood
Female
Glutathione/blood
Glutathione Peroxidase/blood
Glutathione Reductase/blood
Humans
Hydrogen Peroxide/analysis
Lipid Peroxidation
*Oxidative Stress
Placenta/metabolism
Pregnancy
Reactive Oxygen Species/metabolism
Saudi Arabia/epidemiology
Selenium/blood
Antioxidants
Biomarkers
Catalase
Reactive Oxygen Species
Hydrogen Peroxide
Glutathione
Glutathione Peroxidase
Glutathione Reductase
Selenium

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