J Korean Ophthalmol Soc.
2008 Oct;49(10):1665-1670.
Effect of Hydrogen Peroxide-induced Oxidative Stress on the Senescence of Trabecular Meshwork Cells
- Affiliations
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- 1Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Koera. jwkim@cu.ac.kr
Abstract
- PURPOSE
To investigate the effects of oxidative stress on the senescence of trabecular meshwork (TM) cells and the effect of L-ascorbic acid (LAA) against oxidative stress-induced senescence.
METHODS
Primary cultured human TM cells were exposed to 0.05 or 0.1 mM hydrogen peroxide for 30 minutes and incubated for 1 week with or without co-exposure of LAA. Cellular survival, nitrite production, and senescence were assessed with MTT, Griess, and SA-beta-gal assays, respectively.
RESULTS
Hydrogen peroxide decreased cellular survival and NO production accompanied increased cellular senescence. LAA did not prevent hydrogen peroxide-induced senescence.
CONCLUSIONS
Oxidative stress-induced senescence of TM cells may be related to the dysfunction of trabecular meshwork in glaucoma.
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