J Korean Ophthalmol Soc.  2008 Dec;49(12):1989-1995.

Role of Ascorbic Acid Against the Oxidative Stress in Trabecular Meshwork Cells

• Ryu JY
• Kim JW

Abstract

PURPOSE: To investigate the role of L-ascorbic acid (LAA) on the survival and its association with nitric oxide (NO) production against hydrogen peroxide-induced oxidative stress in trabecular meshwork (TM) cells.
METHODS
Primarily cultured human TM cells were exposed to hydrogen peroxide; 10, 100 micrometer for 2 days, or 1 mM single exposure with or without co-exposure of LAA. Cellular survival and nitrite production were assessed with MTT and Griess assays, respectively. Flow cytometry using annexin/PI double staining was performed to evaluate apoptosis.
RESULTS
Hydrogen peroxide decreased cellular survival significantly in a dose-dependent manner accompanied with decreased NO production. However cellular survival and NO production were increased significantly with co-exposure of LAA. Cellular survival and NO were highly correlated. Flow cytometric analysis revealed that LAA inhibited hydrogen peroxide-induced apoptosis.
CONCLUSIONS
LAA demonstrated a cytoprotective effect against the hydrogen peroxide-induced oxidative stress accompanied with increased NO production. The cytoprotective effect of LAA may be mediated by preserving NO in TM cells.

Keyword

Ascorbic acid; Hydrogen peroxide; Nitric oxide; Oxidative stress; Trabecular meshwork cells

MeSH Terms

Ascorbic Acid
Flow Cytometry
Humans
Hydrogen
Hydrogen Peroxide
Nitric Oxide
Oxidative Stress
Trabecular Meshwork
Ascorbic Acid
Hydrogen
Hydrogen Peroxide
Nitric Oxide