J Korean Ophthalmol Soc.  2013 Mar;54(3):490-495. 10.3341/jkos.2013.54.3.490.

Effect of Ascorbic Acid Against the Oxidative Stress-Induced Cellular Senescence in Trabecular Meshwork Cells

• Kim JW
• Kang SH
• Lee KW

• KMID: 2216771
• DOI: http://doi.org/10.3341/jkos.2013.54.3.490

Abstract

PURPOSE
To investigate the effect of L-ascorbic acid (LAA) on oxidative-stress-induced cellular senescence in trabecular meshwork (TM) cells.
METHODS
Primarily cultured human TM cells were exposed to 0, 10, or 100 microM hydrogen peroxide for 7 days with or without co-exposure of LAA. Cellular survival and nitrite production were assessed with MTT and Griess assays, respectively. SA-beta-gal staining was performed to quantify cellular senescence.
RESULTS
Hydrogen peroxide decreased cellular survival, accompanied by decreased nitric oxide (NO) production. These decreases of cellular survival and NO production were abolished by co-exposure of 100 microM LAA. Analysis of SA-beta-gal staining revealed that LAA inhibited hydrogen peroxide-induced cellular senescence by 6.8% (p < 0.05).
CONCLUSIONS
LAA may have a protective effect against the oxidative-stress-induced cellular senescence in TM cells.

Keyword

Ascorbic acid; Oxidative stress; Senescence; Trabecular meshwork cells

MeSH Terms

Aging
Ascorbic Acid
Cell Aging
Humans
Hydrogen
Hydrogen Peroxide
Nitric Oxide
Oxidative Stress
Trabecular Meshwork
Ascorbic Acid
Hydrogen
Hydrogen Peroxide
Nitric Oxide