J Korean Pain Soc.  2001 Dec;14(2):142-149.

Spinal c-fos Expression in a Rat Model of Incisional Pain

Affiliations
  • 1Department of Anesthesiology, Medical School, Chonnam National University, Gwangju, Korea. mhyoon@chonnam.ac.kr
  • 2Department of Pain Medicine, Medical School, Chonnam National University, Gwangju, Korea.
  • 3Department of Anatomy, Medical School, Chonnam National University, Gwangju, Korea.

Abstract

BACKGROUND: The expression of the proto-oncogene c-fos in spinal cord neurons following various noxious stimuli has been demonstrated in numerous studies. However, the pattern of expression of c-fos after incisional stimulus has not been evaluated. This study was designed to examine c-fos expression in an incisional pain model of rats.
METHODS
A 1 cm longitudinal incision was made through the skin, fascia and muscle of the plantar aspect of the hindpaw in enflurane-anesthetized rats. Withdrawal responses were measured using von Frey filaments at areas around the wound before surgery and for the next 48 hours. The expression of c-fos protein in the lumbar spinal cord was examined by immunohistochemistry.
RESULTS
After incision, c-fos was strongly expressed within laminae I, II, III, IV, V and VI ipsilateral to the incision. C-fos positive neurons were detected in the controlateral site, as well.
CONCLUSIONS
These studies suggest that spinal c-fos protein may not be used as a specific marker for spinal nociceptive processing in an incisional pain model.

Keyword

C-fos; Incisional model; Spinal cord; Pain

MeSH Terms

Animals
Fascia
Immunohistochemistry
Models, Animal*
Neurons
Proto-Oncogenes
Rats*
Skin
Spinal Cord
Wounds and Injuries
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