Yeungnam Univ J Med.  2007 Jun;24(1):67-78.

Upregulation of IP-10(CXCL10) mRNA Expression by Interleukin-18

• Kim HY
• Kim HS

Abstract

BACKGROUND: Interleukin-18 (IL-18) is one of the principal inducers of interferon-gamma (IFN-gamma) in lymphocytes.
MATERIALS AND METHODS
The effect of IL-18 on the expression of chemokine IP-10(CXCL10) mRNA in C57BL/6 mouse peritoneal macrophages was studied by using Northern blot analysis, enzyme linked immunosobent assay and electrophoretic mobility shift assay.
RESULTS
IL-18 was determined to exert no direct effect on the expression of IP-10(CXCL10) mRNA. However, IL-18 pretreatment was determined to play a cooperative role in the synergistic induction of LPS-induced IP-10(CXCL10) mRNA expression. The effect associated with IL-18 pretreatment with regard to the synergistic induction of LPS-induced IP-10 (CXCL10) mRNA expression was detected after 16 hr of IL-18 pretreatment, administered prior to LPS stimulation. The pattern of NF-kB binding activity during IL-18 pretreatment with LPS stimulation was found to coincide with the expression of IP-10(CXCL10) mRNA.
CONCLUSION
Although IL-18 alone exerts no direct effect on the expression of chemokine IP-10(CXCL10), a definite period of IL-18 pretreatment induces the synergistic expression of LPS-induced IP-10(CXCL10) mRNA. NF-kB activation is a component of this synergistic effect of IL-18 pretreatment. These results provide useful information, which may facilitate the elucidation of the action mechanisms underlying IL-18 effect on the expression of IP-10(CXCL10) mRNA.

Keyword

IP-10(CXCL10); Interleukin-18; Lipopolysaccharide

MeSH Terms

Animals
Blotting, Northern
Electrophoretic Mobility Shift Assay
Interferon-gamma
Interleukin-18*
Lymphocytes
Macrophages, Peritoneal
Mice
NF-kappa B
RNA, Messenger*
Up-Regulation*
Interferon-gamma
Interleukin-18
NF-kappa B
RNA, Messenger