Korean J Urol.  2011 May;52(5):359-363.

Transplantation of Muscle-Derived Stem Cells into the Corpus Cavernosum Restores Erectile Function in a Rat Model of Cavernous Nerve Injury

Affiliations
  • 1Department of Urology, College of Medicine, The Catholic University of Korea, Seoul, Korea. ksw1227@catholic.ac.kr
  • 2Department of Urology, College of Medicine, The Jeju National University of Korea, Jeju, Korea.
  • 3Division of Molecular and Life Science, Integrative Bioscience and Biotechnology, WCU, POSTECH, Pohang, Korea.

Abstract

PURPOSE
Muscle-derived stem cells (MDSCs) harvested from skeletal muscles have the advantage of providing easier access and do not pose the immunogenic risks of embryonic stem cells. We investigated the effect of intracavernosal transplantation of MDSCs on erectile function in rats with bilateral cavernous nerve injury.
MATERIALS AND METHODS
Adult male white rats underwent experimentation in 3 groups: group I, sham operation; group II, bilateral cavernous nerve injury; group III, bilateral cavernous nerve injury with MDSC injection. MDSCs were harvested from the femoral muscle of rats and were then injected into the cavernosum. Survival of MDSCs and measurement of erectile function was studied after 4 weeks. We checked the intracavernosal pressure (ICP) and obtained penile tissue. The expression of cyclic guanosine monophosphate (cGMP) was analyzed.
RESULTS
Four weeks after transplantation, PKH-26-labeled MDSCs were identified in the cavernosal tissues of group III. Peak ICP and the drop rate of group II were 52+/-8.7 mmHg and 34+/-6.5 mmHg/min, respectively, whereas peak ICP and the drop rate of group III were 97+/-15.6 mmHg and 17+/-4.9 mmHg/min, respectively, showing that erectile function improved after MDSC transplantation (p<0.05). The expression of cGMP was significantly lower in group II (21.9+/-5.8 fmol/well) than in group I and group III (70.2+/-10.3 and 58.9+/-10.5 fmol/well, respectively).
CONCLUSIONS
In a cavernous nerve injury rat model, intracavernosal transplantation of MDSCs showed acceptable survival of MDSCs as well as improvement of erectile function.

Keyword

Erectile dysfunction; Stem cells; Transplantation
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