Nutr Res Pract.  2009 Jun;3(2):84-88.

The inhibition of inflammatory molecule expression on 3T3-L1 adipocytes by berberine is not mediated by leptin signaling

Affiliations
  • 1School of Life Science, Handong Global University, Heunghae-eup, Buk-gu, Pohang, Gyeongbuk 791-708, Korea. msdo@handong.edu

Abstract

In our previous study, we have shown that berberine has both anti-adipogenic and anti-inflammatory effects on 3T3-L1 adipocytes, and the anti-adipogenic effect is due to the down-regulation of adipogenic enzymes and transcription factors. Here we focused more on anti-inflammatory effect of berberine using real time RT-PCR and found it changes expressions of adipokines. We hypothesized that anti-adipogenicity of berberine mediates anti-inflammtory effect and explored leptin as a candidate mediator of this signaling. We studied this hypothesis by western blot analysis, but our results showed that berberine has no effect on the phosphorylations of STAT-3 and ERK which have important roles on leptin signaling. These results led us to conclude that the anti-inflammatory effect of berberine is not mediated by the inhibition of leptin signal transduction. Moreover, we have found that berberine down-regulates NF-kappaB signaling, one of the inflammation-related signaling pathway, through western blot analysis. Taken together, the anti-inflammatory effect of berberine is not mediated by leptin, and berberine induces anti-inflammatory effect independent of leptin signaling.

Keyword

Berberine; inflammation; STAT-3; I-kappaB phosphorylation; 3T3-L1 adipocytes

MeSH Terms

Adipocytes
Adipokines
Berberine
Blotting, Western
Down-Regulation
Inflammation
Leptin
NF-kappa B
Phosphorylation
Signal Transduction
Transcription Factors
Adipokines
Berberine
Leptin
NF-kappa B
Transcription Factors
Full Text Links
  • NRP
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
    DB Error: unknown error