Diabetes Metab J.  2015 Feb;39(1):59-65. 10.4093/dmj.2015.39.1.59.

Myeloperoxidase Is Associated with Insulin Resistance and Inflammation in Overweight Subjects with First-Degree Relatives with Type 2 Diabetes Mellitus

• Gomez Garcia A
• Rodriguez
• Gomez Alonso C
• Ochoa DY
• Alvarez Aguilar C

• KMID: 2280658
• DOI: http://doi.org/10.4093/dmj.2015.39.1.59

Abstract

BACKGROUND
Family history of type 2 diabetes mellitus (T2DM) is one of risk factors for that in future a subject can develop diabetes. Insulin resistance (IR) is important in the pathogenesis of T2DM. There is evidence that oxidative stress plays an important role in the etiology and/or progression of diabetes. Myeloperoxidase (MPO) participates in developing of inflammation. The objective was to investigate if MPO is associated with IR and inflammation in individuals with first-degree relatives of T2DM.
METHODS
Cross-sectional study in 84 overweight individuals with family history of T2DM divided in two groups according to IR, group with IR (homeostasis model assessment [HOMA] > or =2.5; n=43) and control group (CG; HOMA <2.5; n=41). Complete clinical history and a venous blood sample were collected for measuring glucose and lipids profile, insulin, interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), MPO, glutathione reductase (GRd), glutathione peroxidase, and superoxide dismutase.
RESULTS
MPO, TNF-alpha, and IL-6 were higher in patients with IR than in CG (MPO: 308.35 [190.85 to 445.42] vs. 177.35 [104.50 to 279.85], P=0.0001; TNF-alpha: 13.46 [10.58 to 18.88] vs. 9.39 [7.53 to 11.25], P=0.0001; IL-6: 32.93 [24.93 to 38.27] vs. 15.60 [12.93 to 26.27]; P=0.0001, respectively). MPO was associated with IR (rho de Spearman=0.362, P=0.001). In the analysis of lineal regression, MPO predicts IR (beta, 0.263; t, 2.520; P=0.014). In the univariate analysis, MPO had an odds ratio of 9.880 for risk of IR (95% confidence interval, 2.647 to 36.879).
CONCLUSION
MPO had relation with IR and inflammation parameters in overweight subjects with first-degree relatives of T2DM. We need studies on a casual relationship and molecular mechanisms among the increased serum MPO levels, inflammation markers, and IR.

Keyword

First-degree relatives of type 2 diabetes mellitus; Insulin resistance; Myeloperoxidase

MeSH Terms

Cross-Sectional Studies
Diabetes Mellitus, Type 2*
Glucose
Glutathione Peroxidase
Glutathione Reductase
Humans
Inflammation*
Insulin
Insulin Resistance*
Interleukin-6
Odds Ratio
Overweight*
Oxidative Stress
Peroxidase*
Risk Factors
Superoxide Dismutase
Tumor Necrosis Factor-alpha
Glucose
Glutathione Peroxidase
Glutathione Reductase
Insulin
Interleukin-6
Peroxidase
Superoxide Dismutase
Tumor Necrosis Factor-alpha

Figure

• 

  Fig. 1 Odds ratio and (95% confidence interval [CI]) analysis of variables of oxidative stress and inflammation for insulin resistance in overweight subjects with first-degree relatives with type 2 diabetes mellitus. MPO, myeloperoxidase; SOD, superoxide dismutase; GPx, glutathione peroxidase; GRd, gluthathione reductase; IL-6, interleukin-6; TNF-α, tumor necrosis factor-α.

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