Chonnam Med J.
2001 Jun;37(2):159-164.
Development of the Screening Method for Cholesterol Biosynthesis Inhibitors
- Affiliations
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- 1Department of Internal Medicine, Kwangju Veterans Hospital, Kwangju, Korea.
- 2Research Institute of Medical Sciences, Chonnam National University, Kwangju, Korea.
Abstract
- Hypercholesterolemia is a major risk factor in cardiovascular diseases. We have tried to develop a simple, sensitive method for screening the materials expected to have inhibitory effects on cholesterol biosynthesis in liver. We used 14C-acetic acid as a precursor of cholesterol biosynthesis in human hepatocytes and separated C27 sterols on silica gel plate and analyzed by phosphor image analysis system. The inhibitory effects of mevastatin and LDAO (lauryldimethyllamine N-oxide) on cholesterol biosynthesis were clearly detected. We screened sixteen samples of plant extracts and six samples of microbial extracts. Two of them appeared to have the inhibitory effect on cholesterol biosynthesis in human hepatocytes. These results suggest that this assay system could be used in screening various materials to develop new drugs lowering the cholesterol concentration in serum.
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