Ann Dermatol.  2013 Aug;25(3):315-320. 10.5021/ad.2013.25.3.315.

A Novel Compound Rasatiol Isolated from Raphanus sativus Has a Potential to Enhance Extracellular Matrix Synthesis in Dermal Fibroblasts

• Roh SS
• Park SB
• Park SM
• Choi BW
• Lee MH
• Hwang YL
• Kim CH
• Jeong HA
• Kim CD
• Lee JH

• KMID: 2265871
• DOI: http://doi.org/10.5021/ad.2013.25.3.315

Abstract

BACKGROUND
The fibrous proteins of extracellular matrix (ECM) produced by dermal fibroblast contributes to the maintenance of connective tissue integrity.
OBJECTIVE
This study is carried out to identify the bioactive ingredient from natural products that enhances ECM production in dermal fibroblasts.
METHODS
Bioassay-directed fractionation was used to isolate the active ingredient from natural extracts. The effects of rasatiol (isolated from Raphanus sativus) on ECM production in primary cultured human dermal fibroblasts was investigated by enzyme linked immunosorbent assay and western blot analysis.
RESULTS
Rasatiol accelerated fibroblast growth in a dose-dependent manner and increased the production of type 1 collagen, fibronectin and elastin. Phosphorylation of p42/44 extracellular signal-regulated kinase, p38 mitogen-activated protein kinase, and Akt was remarkably increased by rasatiol, indicating that enhanced ECM production is linked to the activation of intracellular signaling cascades.
CONCLUSION
These results indicate that rasatiol stimulates the fibrous components of ECM production, and may be applied to the maintenance of skin texture.

Keyword

Extracellular matrix; Fibroblasts; Rasatiol

MeSH Terms

Biological Agents
Blotting, Western
Collagen Type I
Connective Tissue
Elastin
Enzyme-Linked Immunosorbent Assay
Extracellular Matrix
Fibroblasts
Fibronectins
Humans
Phosphorylation
Phosphotransferases
Protein Kinases
Raphanus
Scleroproteins
Skin
Biological Agents
Collagen Type I
Elastin
Fibronectins
Phosphotransferases
Protein Kinases
Scleroproteins